Fluorescence turn-on sensing of protein based on mannose functionalized perylene bisimides and its fluorescence imaging
Fluorescence turn-on sensing of protein based on mannose functionalized perylene bisimides and its fluorescence imaging
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基于甘露糖功能化苝双酰亚胺的蛋白质荧光开启传感及其荧光成像
DOI：
10.1016/j.bios.2014.02.038
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发表时间：
2014-08-15
影响因子：
12.6
通讯作者：
Li, Xiao-Liu
中科院分区：
文献类型：
--
作者：
Wang, Ke-Rang;An, Hong-Wei;Li, Xiao-Liu
设计并合成了一种新的水溶性二萘嵌苯双酰亚胺PBI-12-Man糖簇，并通过荧光光谱和圆二色光谱研究了其与刀豆球蛋白A（ConA）的特异性和选择性结合，结果表明，该糖簇与ConA具有较强的结合亲和力，其甘露糖单体的结合常数为8.2 × 10（5）M-1，比相应的单糖配体高两个数量级。最有趣的是，PBI-12-Man在与ConA结合时观察到荧光增强，这是由于碳水化合物-蛋白质相互作用诱导的PBI-12-Man的自组装的解聚集，并且对巨噬细胞荧光增强的进一步研究表明，PBI-12-Man作为生物相容性试剂具有细胞表面甘露糖受体的荧光成像。这种基于碳水化合物-蛋白质相互作用的蛋白质的荧光开启感测将促进新的蛋白质特异性荧光探针的开发，用于活细胞条件下的诊断和分子成像。(C)2014爱思唯尔有限公司版权所有。
A new water-soluble glycocluster based on perylene bisimides PBI-12-Man has been designed and synthesized, and its specific and selective binding property with Concanavalin A (Con A) has been investigated by fluorescence spectroscopy and circular dichroism (CD) spectroscopy, which showed strong binding affinity for Con A with the binding constant of 8.2 x 10(5) M-1 for monomeric mannose unit, two orders of magnitude higher than the corresponding monosaccharide ligand. Most interestingly, a fluorescence enhancement of PBI-12-Man was observed upon binding with Con A because of deaggregation of the self-assembly of PBI-12-Man induced by carbohydrate-protein interaction, and the further study of the fluorescence enhancement with macrophage cells showed that PBI-12-Man as a biocompatible agent had fluorescence imaging of the surface mannose receptor of the cells. Such fluorescence turn-on sensing of protein based on carbohydrate-protein interactions would facilitate the development of new protein-specific fluorescent probe for diagnosis and molecular imaging under live cell conditions. (C) 2014 Elsevier B.V. All rights reserved.