AFM Study for Morphological Characteristics and Biomechanical Properties of Human Cataract Anterior Lens Capsules
AFM Study for Morphological Characteristics and Biomechanical Properties of Human Cataract Anterior Lens Capsules
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DOI：
10.1002/sca.21001
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发表时间：
2012-07-01
期刊：
影响因子：
--
通讯作者：
Park, Young-Guk
中科院分区：
文献类型：
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作者：
Choi, Samjin;Lee, Hui-Jae;Park, Young-Guk
本研究的目的是利用原子力显微镜定量研究非白内障和白内障患者前晶状体囊（ALCs）的形态（表面粗糙度）和生物力学特性（杨氏模量）。研究了8例老年性白内障患者（72 +/- 13岁）超声乳化术中获得的alc在脱水和脱水条件下的表现。白内障组可见晶状体上皮细胞增生，细胞结构单一，直径12.54 +/- 4.31 μ m，高度0.23 +/- 0.04 μ m，对照组未见晶状体上皮细胞增生。观察到大量的假阳性钙化是由干盐溶液残留物的沉积引起的。白内障组ALCs前侧壁表面粗糙度（382.06 nm, p = 0.001）明显高于对照组，后侧壁表面粗糙度（353.79 nm, p = 0.001）明显低于对照组。白内障组的杨氏模量（69.52 kPa, p = 0.001）明显高于对照组，与ALC侧无关。因此，本研究提供了一种通过纳米尺度分辨率显微技术来研究人类ALCs的纳米结构特征和生物力学特性的新方法，具有重要意义。中文信息学报，2012。(c) 2012 Wiley期刊有限公司
The aim of this study was to quantitatively investigate the morphologies (surface roughness) and biomechanical properties (Young's modulus) of human anterior lens capsules (ALCs) for noncataract and cataract groups using atomic force microscopy. Eight human ALCs obtained during phacoemulsification from patients with senile cataracts (72 +/- 13 years) were investigated in both the hydrated and dehydrated conditions. The cataract group showed clearly the proliferated lens epithelial cells (LECs) with a monomorphic cell structure, a diameter of 12.54 +/- 4.31 mu m, and a height of 0.23 +/- 0.04 mu m, whereas the control group showed no LECs. A substantial amount of false-positive calcification was observed caused by the deposition of remnants of dried salt solution. Cataract group showed significantly higher surface roughness (382.06 nm, p = 0.001) than control group in the anterior side of ALCs, whereas cataract group showed significantly lower surface roughness (353.79 nm, p = 0.001) than control group in their posterior side. Cataract group showed significantly higher Young's modulus (69.52 kPa, p = 0.001) compared to the control group, regardless of the ALC side. Therefore, it is significant that this study provides a new method to examine the nanostructural characteristic and biomechanical property of human ALCs through a nanometer-scale resolution microscopy technique. SCANNING 34: 247-256, 2012. (c) 2012 Wiley Periodicals, Inc.