Interleukin (IL)-6 modulates transforming growth factor-beta expression in skin and dermal fibroblasts from IL-6-deficient mice.
Interleukin (IL)-6 modulates transforming growth factor-beta expression in skin and dermal fibroblasts from IL-6-deficient mice.
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DOI：
10.1111/j.1365-2133.2009.09215.x
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发表时间：
2009-08
期刊：
影响因子：
--
通讯作者：
Gallucci RM
中科院分区：
文献类型：
--
作者：
Luckett-Chastain LR;Gallucci RM
Interleukin (IL-6) and transforming growth factor (TGF)-β have been shown to play a role in skin development and maintenance. A link between these two cytokines has yet to be identified and therefore in this study we investigated the modulation of TGF-β1 and TGF-β type 2 receptor (TGF-βR2) by IL-6 in skin. An IL-6 knockout (IL-6KO) fibroblast-populated lattice model and intradermal injections of IL-6 into unwounded IL-6KO mice were used to investigate the direct effects of IL-6 treatment on TGF-β and TGF-βR2 expression and to determine the signalling mechanism. In addition, IL-6KO and C57BL/6 control mice were wounded by a 4-mm punch biopsy to monitor expression of TGF-β1 and TGF-βR2 within a wound over time. The expression of TGF-β1 and TGF-βR2 was assessed by real-time quantitative polymerase chain reaction, enzyme-linked immunosorbent assay and immunohistology. Recombinant IL-6 treatment of IL-6KO lattices and intradermal injections of IL-6 showed a significant induction of TGF-β1 mRNA and protein, with TGF-β1 expression localized in the dermis, while TGF-βR2 expression was primarily in the epidermis in IL-6KO mice. During healing, the expression of TGF-β1 and TGF-βR2 mRNA was significantly greater in unwounded and 7-day-old wounds from wild-type mice; however, protein expression did not differ. Treatment with signal transduction inhibitors indicated that IL-6 modulates TGF-β through a mitogen-activated protein kinase/extracellular signal-regulated kinase (Mapk/Erk)-dependent mechanism. These studies indicate that IL-6 has the ability to modulate the expression of TGF-β and TGF-βR2 to varying degrees in the skin, which may provide a possible mechanism for defining the role of IL-6 in skin maintenance and a new association of IL-6 with TGF-β in pathologies associated with fibrosis.
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DOI：
10.1073/pnas.92.17.7686
发表时间：
1995-08-15
影响因子：
11.1
作者：
DUDLEY, DT;PANG, L;SALTIEL, AR
通讯作者：
SALTIEL, AR
影响因子：
25.7
作者：
ANNONI, G;WEINER, FR;ZERN, MA
通讯作者：
ZERN, MA
影响因子：
8
作者：
Decker, T;Kovarik, P
通讯作者：
Kovarik, P
影响因子：
6.5
作者：
Gallucci, Randle M.;Lee, Eric G.;Tomasek, James J.
通讯作者：
Tomasek, James J.
DOI：
10.1016/j.bbrc.2006.12.204
发表时间：
2007-03-09
影响因子：
3.1
作者：
Ghosh, Asish K.;Mori, Yasuji;Varga, John
通讯作者：
Varga, John