RcoA has pleiotropic effects on Aspergillus nidulans cellular development
RcoA has pleiotropic effects on Aspergillus nidulans cellular development
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DOI：
10.1046/j.1365-2958.2001.02332.x
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发表时间：
2001-03-01
影响因子：
3.6
通讯作者：
Keller, N
中科院分区：
文献类型：
--
作者：
Hicks, J;Lockington, RA;Keller, N
细粒曲霉rcoA编码WD重复蛋白家族的一个成员。RcoA蛋白与该蛋白家族的其他成员（包括Saccharomyces cerevisiae Tup1p和Neurospora crassa RCO1）具有序列相似性。Tup1p参与一系列功能的负调控，包括碳分解代谢抑制。RCO1在调节多效性发育过程中起作用，但不抑制碳分解代谢。在a . nidulans中，rcoA （Delta rcoA）隐性突变的缺失导致营养生长、无性孢子产生和sterigmatocystin （ST）生物合成的严重缺陷。在Delta rcoA菌株中，无性和ST通路特异性调控基因brlA和aflR的表达被延迟（brlA）或消除（aflR），而调节brlA和aflR表达的信号转导基因（即flbA、fluG或fadA）未被表达。在Delta rcoA菌株中，过表达aflR不能挽救aflR下游靶标的正常表达。在Delta rcoA菌株中，依赖crea的碳分解物对淀粉和乙醇利用的抑制作用仅受到微弱影响。RcoA在发育、营养生长和ST产生中的作用较强，而在碳分解代谢抑制中的作用相对较弱，这与RCO1在N. crassa中的作用相似。
Aspergillus nidulans rcoA encodes a member of the WD repeat family of proteins. The RcoA protein shares sequence similarity with other members of this protein family, including the Saccharomyces cerevisiae Tup1p and Neurospora crassa RCO1. Tup1p is involved in negative regulation of an array of functions including carbon catabolite repression. RCO1 functions in regulating pleiotropic developmental processes, but not carbon catabolite repression. In A. nidulans, deletion of rcoA (Delta rcoA), a recessive mutation, resulted in gross defects in vegetative growth, asexual spore production and sterigmatocystin (ST) biosynthesis. Expression of the asexual and ST pathway-specific regulatory genes, brlA and aflR, respectively, but not the signal transduction genes (i.e. flbA, fluG or fadA) regulating brlA and aflR expression was delayed (brlA) or eliminated (aflR) in a Delta rcoA strain. Overexpression of aflR in a Delta rcoA strain could not rescue normal expression of downstream targets of AflR. CreA-dependent carbon catabolite repression of starch and ethanol utilization was only weakly affected in a Delta rcoA strain. The strong role of RcoA in development, vegetative growth and ST production, compared with a relatively weak role in carbon catabolite repression, is similar to the role of RCO1 in N. crassa.