Amino acids regulate the intracellular trafficking of the general amino acid permease of Saccharomyces cerevisiae
Amino acids regulate the intracellular trafficking of the general amino acid permease of Saccharomyces cerevisiae
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DOI：
10.1073/pnas.232591899
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发表时间：
2002-11-12
影响因子：
11.1
通讯作者：
Kaiser, CA
中科院分区：
文献类型：
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作者：
Chen, EJ;Kaiser, CA
酿酒酵母总氨基酸渗透酶Gap1p向质膜的转运受生长介质中氮源的质量调节。为了确定不同的氮源如何控制Gap1p分选，我们发现GDH1和GLN1的突变导致谷氨酸和谷氨酰胺合成途径的通量降低，导致Gap1p对质膜的分选增加。相反，MKS1的缺失增加了谷氨酸和谷氨酰胺的合成，减少了Gap1p对质膜的分选。谷氨酸和谷氨酰胺在调节Gap1p分选方面并不罕见，因为在生长介质中添加所有天然氨基酸和许多氨基酸类似物会导致Gap1p对液泡的分选增加。重要的是，氨基酸具有向液泡发送Gap1p分类信号的能力，无论它们是否可以用作氮源。最后，我们发现雷帕霉素不影响Gap1p排序，表明Gap1p排序不直接受TOR途径的影响。综上所述，这些数据表明，氨基酸是将Gap1p分拣到液泡的信号，并意味着氮调节的Gap1p分拣机制对氨基酸类化合物做出反应，而不是对与特定氮源上生长相关的整体营养状况做出反应。
The delivery to the plasma membrane of the general amino acid permease, Gap1p, of Saccharomyces cerevisiae is regulated by the quality of the nitrogen source in the growth medium. In an effort to define how different nitrogen sources control Gap1p sorting, we find that mutations in GDH1 and GLN1 that decrease the flux through the glutamate and glutamine synthesis pathways result in increased Gap1p sorting to the plasma membrane. Conversely, deletion of MKS1, which increases glutamate and glutamine synthesis, decreases Gap1p sorting to the plasma membrane. Glutamate and glutamine are not unusual in their ability to regulate Gap1p sorting, because the addition of all natural amino acids and many amino acid analogs to the growth medium results in increased Gap1p sorting to the vacuole. Importantly, amino acids have the capacity to signal Gap1p sorting to the vacuole regardless of whether they can be used as a source of nitrogen. Finally, we show that rapamycin does not affect Gap1p sorting, indicating that Gap1p sorting is not directly influenced by the TOR pathway. Together, these data show that amino acids are a signal for sorting Gap1p to the vacuole and imply that the nitrogen-regulated Gap1p sorting machinery responds to amino acid-like compounds rather than to the overall nutritional status associated with growth on a particular nitrogen source.