Increased plasma mRNAs of placenta-specific 1 (PLAC1) and glial cells-missing 1 (GCM1) in mothers with pre-eclampsia.
Increased plasma mRNAs of placenta-specific 1 (PLAC1) and glial cells-missing 1 (GCM1) in mothers with pre-eclampsia.
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发表时间：
2006-03
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通讯作者：
N. Fujito;O. Samura;N. Miharu;M. Tanigawa;Maki Hyodo;Y. Kudo
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作者：
N. Fujito;O. Samura;N. Miharu;M. Tanigawa;Maki Hyodo;Y. Kudo
在这项研究中，我们研究了是否定量分析胎盘mRNA在母体血浆中提供了一种方法来监测胎盘状态。我们测量了人绒毛膜促性腺激素β亚基（β hCG）和人胎盘催乳素（hPL）mRNA的血浆浓度，如以前报道的mRNA和妊娠相关血浆蛋白A（PAPP-A），胎盘特异性1（PLAC 1）和神经胶质细胞缺失1（GCM 1）mRNA，这些mRNA在正常妊娠过程中尚未测量。首先，在不同时间从健康孕妇获得外周血以阐明胎盘mRNA的时间过程。其次，从患有先兆子痫的妇女和胎龄匹配的对照组中获得血液，以检查胎盘mRNA在先兆子痫中是否发生变化。从这些样品中分离血浆用于提取RNA，然后进行逆转录聚合酶链反应分析。先兆子痫受试者血浆中PLAC 1和GCM 1 mRNA的中位浓度分别为1625和2141拷贝/ml，显著高于对照组的195和881拷贝/ml（Mann-Whitney检验，p<0.001）。先兆子痫组和对照组之间的hPL、β hCG或PAPP-A mRNA浓度无显著差异。血浆PLAC 1和GCM 1 mRNA作为先兆子痫的非侵入性可测量的分子标记物似乎很有前途。
In this study we have investigated whether quantitative analysis of placental mRNAs in maternal plasma provides a way to monitor placental status. We measured plasma concentrations of human chorionic gonadotropin beta-subunit (betahCG) and human placental lactogen (hPL) mRNAs as previously reported mRNAs and pregnancy associated plasma protein A (PAPP-A), placenta-specific 1 (PLAC1) and glial cells-missing 1 (GCM1) mRNAs, which have not been measured during the course of normal pregnancy. Firstly, peripheral blood was obtained at various times from healthy pregnant women to clarify the time course of placental mRNAs. Secondly, blood was obtained from women with pre-eclampsia and gestational age-matched controls to examine whether placental mRNAs change in pre-eclampsia. Plasma was separated from these samples for extraction of RNA, followed by reverse transcription polymerse chain reaction analysis. Median concentrations of PLAC1 and GCM1 mRNA in plasma of pre-eclamptic subjects respectively were 1625 and 2141 copies/ml, significantly higher than 195 and 881 copies/ml, the values for controls (Mann-Whitney test, p<0.001). No significant difference was seen in hPL, betahCG, or PAPP-A mRNA concentration between pre-eclamptic and control groups. Plasma PLAC1 and GCM1 mRNAs appear promising as noninvasively measurable molecular markers for pre-eclampsia.