Ultrastructure of initial nasal process cell fusion in spontaneous and 6-aminonicotinamide-induced mouse embryo cleft lip.
Ultrastructure of initial nasal process cell fusion in spontaneous and 6-aminonicotinamide-induced mouse embryo cleft lip.
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自发和 6-氨基烟酰胺诱导的小鼠胚胎唇裂初始鼻突细胞融合的超微结构。
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
L. Ohannessian
中科院分区：
文献类型：
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作者：
D. Trasler;L. Ohannessian
研究了以下类型小鼠胚胎内侧和外侧鼻突初始融合过程中细胞超微结构的差异：A/J 具有 12% 唇裂 (CL)，CL/Fr 具有 23% CL——两种唇裂易感品系，CL/Fr 6-氨基烟酰胺 (6AN) 处理的 (94% CL) 和来自 C57BL/6 品系的对照 (0% CL) 和舞者股票 (0% CL)。在显示初始接触和融合的上皮细胞中，A/J 和 CL/Fr 菌株与对照之间没有发现可检测到的差异。对照中未接触的上皮表面以及正在形成的裂口处是光滑的。接近或接触的细胞具有细胞突起、细胞间连接、桥粒和微丝，表明对位的上皮之间有牢固的接触。据推测，自发性唇裂是由于面部形状导致一些胚胎接触失败，而在其他胚胎中，接触融合是正常的。这些数据支持了这一观点。然而，经过 6AN 处理的胚胎的情况有所不同。一些经过 6AN 处理的胚胎表现出异常接触，显得位置不正且脆弱。致畸剂还导致细胞死亡增加以及上皮和间质更致密。因此，6AN 诱导的唇裂可能是由于上皮细胞变化和/或鼻突尺寸减小所致。
A search was made for cell ultrastructure differences in the initial fusion process of the medial and lateral nasal processes in mouse embryos of the following types: A/J with 12% cleft lip (CL), CL/Fr with 23% CL--both cleft-lip-predisposed strains, CL/Fr 6-aminonicotinamide (6AN)-treated (94% CL) and controls from the C57BL/6 strain (0% CL) and dancer stock (0% CL). No detectable differences were found between the A/J and CL/Fr strains and the controls in the epithelial cells showing initial contact and fusion. Epithelial surfaces not in contact in controls and where clefts were developing were smooth. Cells approaching or in contact had cell projections, intercellular junctions, desmosomes, and microfilaments demonstrating firm contact between the apposed epithelia. It has been postulated that spontaneous cleft lip was due to a predisposing face shape bringing about a failure of contact in some embryos and in others where contact was achieved fusion was normal. These data support this view. The situation, however, in 6AN-treated embryos is different. A few 6AN-treated embryos showed abnormal contact that appeared malpositioned and tenuous. The teratogen also caused increased cell death and a denser epithelium and mesenchyme. Thus 6AN-induced cleft lip could be due to the epithelial cell changes and/or to the reduction in size of the nasal processes.
胚胎面部上皮的积极作用：形态发生中细胞事件的新证据。
DOI：
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发表时间：
1981
期刊：
Journal of embryology and experimental morphology
影响因子：
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作者：
Millicovsky,G;Johnston,MC
通讯作者：
Johnston,MC