Amperometric detection of catechols in capillary zone electrophoresis with normal and micellar solutions.
Amperometric detection of catechols in capillary zone electrophoresis with normal and micellar solutions.
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DOI：
10.1021/ac00154a015
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发表时间：
1988-02
影响因子：
7.4
通讯作者：
R. A. Wallingford;A. Ewing
中科院分区：
文献类型：
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作者：
R. A. Wallingford;A. Ewing
最近推出的一种用于将毛细管区带电泳与柱外电流检测相连接的系统，已在小孔径色谱柱上与普通溶液和胶束溶液进行了演示。使用较小内径的色谱柱和碳纤维电化学检测器可以提高分离效率并降低检测限。在 26-Miti-ld 色谱柱上，多种儿茶酚的分离效率超过 400 000 个理论塔板，且检测遗漏率低于飞摩尔。为了提高非离子溶质的选择性，采用胶束溶液基于颗粒的分布来分离溶质。检查了胶束溶液中中性和阳离子儿茶酚的电化学，以表征胶束溶液电化学检测的有用性。演示了在 52-jitm-ld 色谱柱上使用胶束溶液对儿茶酚混合物进行电流检测。当使用胶束溶液时，检测到的儿茶酚的最低量低于 20 fmol。Mikkers 等人介绍了毛细管中的区带电泳。 1979 年 (1) 并由 Jorgenson 和同事 (2-4) 在 20 世纪 80 年代初改进。毛细管区带电泳 (CZE) 已发展成为一种分离极少量离子化化合物的高效方法。使用该技术通常可以获得大于 105 个理论塔板的分离效率。 CZE 已被证明是一种极其强大的液相分离技术
A recently Introduced system for Interfacing capillary zone eletrophoresls with off-column amperometric detection Is demonstrated on smaft-bore columns with normal and micellar solutions. The use of smaller Inner diameter columns with a carbon-fiber electrochemical detector allows for Increased separation efficiency and decreased detection limits. On a 26-Miti-ld column, separation efficiencies of greater than 400 000 theoretical plates and subfemtomole detection Omits have been obtained for several catechols. In order to en-hance the selectivity for nonlonlc solutes, micellar solutions were employed to aHow separation of solutes based on partltlohlng into the ndceHes. The electrochemistry of neutral and cationic catechols In mtcelar solutions was examined In order to characterize the usefulness of electrochemical detection with micellar solutions. Amperometric detection with micellar solutions Is demonstrated for a mixture of catechols on a 52-jitm-ld column. Minimum amounts of catechol detected were less than 20 fmol when using micellar solutions.Zone electrophoresis in capillaries was introduced by Mikkers et al. in 1979 (1) and was refined by Jorgenson and co-workers (2-4) in the early 1980s. Capillary zone electrophoresis (CZE) has developed into a highly efficient means of separatingvery small amounts of ionizedcompounds. Separation efficiencies of greater than 105 theoretical plates are often obtained with this technique. CZE has been shown to be an extremely powerful liquid-phase separation technique