miR-19a and miR-19b Overexpression in Gliomas
miR-19a and miR-19b Overexpression in Gliomas
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miR-19a 和 miR-19b 在胶质瘤中过度表达
DOI：
10.1007/s12253-013-9653-x
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发表时间：
2013-10-01
影响因子：
2.8
通讯作者：
Pu, Peiyu
中科院分区：
文献类型：
--
作者：
Jia, Zhifan;Wang, Kun;Pu, Peiyu
星形细胞胶质瘤是最常见的人类原发性脑肿瘤，预后较差。 MicroRNA (miR) 在神经胶质瘤中经常失调，并发挥致癌或肿瘤抑制作用。在我们之前的研究中，我们通过 microRNA 阵列发现恶性胶质瘤细胞系中 miR-19a 和 miR-19b 上调。为了进一步验证这一发现，本研究通过qRT-PCR和原位杂交（ISH）在8个恶性胶质瘤细胞系、43个新鲜切除的胶质瘤样本和75个不同恶性级别的存档石蜡包埋胶质瘤标本中检测了miR-19a和miR-19b的表达。结果表明，miR-19a和miR-19b在胶质瘤细胞系和星形细胞胶质瘤组织中过表达，且其表达水平与肿瘤分级呈正相关。此外，通过荧光素酶测定，肿瘤抑制基因 PTEN 被鉴定为 miR-19a 和 miR-19b 的靶标。据推测，miR-19a和miR-19b可能至少部分通过PTEN的负调控在胶质瘤发生中发挥致癌作用，并且miR 19a和miR-19b参与的胶质瘤发生的分子机制应进一步研究。
Astrocytic gliomas are the most common type of human primary brain tumors with poor prognosis. MicroRNAs(miRs) are frequently deregulated in gliomas and play an oncogenic or tumor suppressor role. In our previous study we found that miR-19a and miR-19b were up-regulated in malignant glioma cell lines by microRNA array. For further validation of this finding, the expression of miR-19a and miR-19b was detected by qRT-PCR and in situ hybridization(ISH) in 8 malignant glioma cell lines, 43 freshly resected glioma samples and 75 archival paraffin embedded glioma specimens with different grades of malignancy in the present study. The results demonstrate that miR-19a and miR-19b are overexpressed in glioma cell lines and astrocytic glioma tissues, and their expression level is positively correlated with tumor grades. Additionally, the tumor suppressor gene PTEN is identified as the target of miR-19a and miR-19b by Luciferase assay. It is speculated that miR-19a and miR-19b may have an oncogenic role in gliomagenesis at least partially via the negative regulation of PTEN and the molecular mechanism of gliomagenesis in which miR 19a and miR-19b involved should be investigated further.