Distributions of ectomycorrhizal and foliar endophytic fungal communities associated with Pinus ponderosa along a spatially constrained elevation gradient
Distributions of ectomycorrhizal and foliar endophytic fungal communities associated with Pinus ponderosa along a spatially constrained elevation gradient
复制标题
DOI：
10.1002/ajb2.1072
复制
发表时间：
2018-04-01
影响因子：
3
通讯作者：
Arnold, A. Elizabeth
中科院分区：
文献类型：
--
作者：
Bowman, Elizabeth A.;Arnold, A. Elizabeth
研究前提：了解植物共生真菌的分布对于预测对环境变化的反应非常重要。许多针叶树的根和叶内都有外生菌根真菌（EM）和叶内生真菌（FE）。我们研究了如何EM和FE与美国黄松的丰富度，多样性和社区结构的空间限制的海拔梯度，穿越四个植物群落，4摄氏度的年平均温度，和15厘米的年平均降水量的变化。我们在沿着635米的海拔梯度，包括9.8公里的地理距离的10个地点，63个个体的黄松。我们使用标准方法来表征每个真菌组（从根尖扩增和测序EM;从叶子分离和测序FE）。关键结果：EM的丰度和多样性在不同地点是相似的，但最常见的EM的群落组成和分布随海拔而不同（即，气候、土壤化学和植物群落）。FE的Abbands和组成并没有随海拔而变化，但多样性在中高海拔达到峰值。结论：我们的研究结果表明EM与气候，土壤化学和植物群落之间存在相对紧密的联系，FE与这些因素的联系较少，可能会说明基于文化的方法的局限性，但更有可能反映了我们的研究所涵盖的小空间尺度。未来的工作应考虑类似的方法来表征这些功能组，和额外的断面，以了解EM和FE的环境因素，可能会改变气候变化的功能的关系。
PREMISE OF THE STUDY: Understanding distributions of plant-symbiotic fungi is important for projecting responses to environmental change. Many coniferous trees host ectomycorrhizal fungi (EM) in association with roots and foliar endophytic fungi (FE) in leaves. We examined how EM and FE associated with Pinus ponderosa each vary in abundance, diversity, and community structure over a spatially constrained elevation gradient that traverses four plant communities, 4 degrees C in mean annual temperature, and 15 cm in mean annual precipitation.METHODS: We sampled 63 individuals of Pinus ponderosa in 10 sites along a 635 m elevation gradient that encompassed a geographic distance of 9.8 km. We used standard methods to characterize each fungal group (amplified and sequenced EM from root tips; isolated and sequenced FE from leaves).KEY RESULTS: Abundance and diversity of EM were similar across sites, but community composition and distributions of the most common EM differed with elevation (i.e., with climate, soil chemistry, and plant communities). Abundance and composition of FE did not differ with elevation, but diversity peaked in mid-to-high elevations.CONCLUSIONS: Our results suggest relatively tight linkages between EM and climate, soil chemistry, and plant communities.That FE appear less linked with these factors may speak to limitations of a culture-based approach, but more likely reflects the small spatial scale encompassed by our study. Future work should consider comparable methods for characterizing these functional groups, and additional transects to understand relationships of EM and FE to environmental factors that are likely to shift as a function of climate change.