Fast Single-Cell Patterning for Study of Drug-Induced Phenotypic Alterations of HeLa Cells Using Time-of-Flight Secondary Ion Mass Spectrometry
Fast Single-Cell Patterning for Study of Drug-Induced Phenotypic Alterations of HeLa Cells Using Time-of-Flight Secondary Ion Mass Spectrometry
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DOI：
10.1021/acs.analchem.6b03170
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发表时间：
2016-12-20
影响因子：
7.4
通讯作者：
Wu, Hongkai
中科院分区：
文献类型：
--
作者：
Huang, Lu;Chen, Yin;Wu, Hongkai
建立了一种简便的单细胞模式化（ScP）方法，并将其与飞行时间二次离子质谱（TOF-SIMS）相结合，用于研究药物诱导的细胞表型改变。结合微图案化聚二甲基硅氧烷（PDMS）模板膜和离心辅助细胞捕获用于制备表面上的单细胞微阵列，其表现出高位点占有率（>90%）和单细胞分辨率（>97%）。飞行时间二次离子质谱是一种表面灵敏的质谱技术，在生物学研究中的应用越来越广泛。在这里，我们首次展示了其在高通量单细胞分析中的成功应用。应用飞行时间二次离子质谱（TOF-SIMS）研究药物诱导的HeLa细胞凋亡早期表型改变。采用主成分分析法（PCA）对变异的主要分子来源进行了分析。
A facile single-cell patterning (ScP) method was developed and integrated with time-of-flight secondary ion mass spectrometry (TOF-SIMS) for the study of drug-induced cellular phenotypic alterations. Micropatterned poly-(dimethylsiloxane) (PDMS) stencil film and centrifugation assisted cell trapping were combined for the preparation of on surface single-cell microarrays, which exhibited both high site occupancy (>90%) and single-cell resolution (>97%). TOF-SIMS is a surface-sensitive mass spectrometry and is increasingly utilized in biological studies. Here we demonstrated, for the first time, its successful application in high throughput single-cell analysis. Drug-induced phenotypic alterations of HeLa cells in the early stage of apoptosis were investigated using TOF-SIMS. The major molecular sources of variations were analyzed by principle component analysis (PCA).