Stimulation of adult chondrocyte metabolism by a thyroid-derived factor.
Stimulation of adult chondrocyte metabolism by a thyroid-derived factor.
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甲状腺衍生因子刺激成体软骨细胞代谢。
DOI：
10.1002/jor.1100080211
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Smith,RL
中科院分区：
文献类型：
--
作者：
Jones,DG;Smith,RL
本文报道了加入部分纯化的牛甲状腺降钙素、甲状腺降钙素对成年牛关节软骨细胞的影响。在低血清(0.5%)培养条件下，甲状腺降钙素可刺激软骨细胞增殖4倍。在无血清培养条件下，甲状腺降钙素可刺激细胞增殖2倍以上。在无血清高密度培养条件下，甲状腺降钙素可刺激60%的软骨细胞糖胺聚糖(GAG)合成。细胞相关放射性增加了两倍。与甲状腺降钙素相反，添加人和鲑鱼降钙素多肽以及甲状腺激素T3和T4对成人软骨细胞增殖或GAG合成没有影响。这里报道的数据表明，存在一种独立于降钙素多肽或甲状腺激素的甲状腺衍生因子，它可以刺激成人关节软骨细胞的代谢。
This paper reports the effects of adding partially purified bovine thyroid calcitonin, thyrocalcitonin, to adult bovine articular cartilage cells. Thyrocalcitonin stimulated chondrocyte proliferation fourfold under low serum (0.5%) culture conditions. In serum‐free medium, thyrocalcitonin stimulated cell proliferation more than twofold. With high‐density cultures in serum‐free medium, chondrocyte glycosaminoglycan (GAG) synthesis was stimulated 60% by thyrocalcitonin. Cell‐associated radioactivity was increased twofold. In contrast to thyrocalcitonin, addition of human and salmon calcitonin peptides as well as the thyroid hormones T3and T4had no effect on adult cartilage cell proliferation or GAG synthesis. The data reported here suggest the existence of a thyroid‐derived factor, independent of calcitonin peptides or thyroid hormones, which stimulates adult articular chondrocyte metabolism.