An early commitment to expression of a particular TCRVbeta chain on CD8(+) T cells responding to attenuated Plasmodium berghei sporozoites is maintained following challenge with infectious sporozoites.
An early commitment to expression of a particular TCRVbeta chain on CD8(+) T cells responding to attenuated Plasmodium berghei sporozoites is maintained following challenge with infectious sporozoites.
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在感染性子孢子攻击后，CD8(+) T 细胞对减毒伯氏疟原虫子孢子作出反应的早期承诺表达特定的 TCRVbeta 链。
DOI：
10.1111/j.1365-3024.2010.01220.x
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发表时间：
2010
影响因子：
2.2
通讯作者：
Krzych,U
中科院分区：
文献类型：
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作者：
Lumsden,JM;Cranmer,MA;Krzych,U
辐照的伯氏疟原虫孢子体（Pbγ - spz）对小鼠的保护作用与红细胞外期Ags特异性CD8+T细胞有关，肝内记忆CD8+T细胞与持久保护有关。然而，保护性CD8+T细胞的Ag特异性在很大程度上仍然未知。在这项研究中，我们描述了在γ - spz免疫期间和传染性Pb孢子虫攻击后肝内CD8+T细胞对TCR Vβ的使用。naïve （TN）和中枢记忆（TCM） CD8+T细胞库在个体小鼠之间具有多样性和保守性，并且不随免疫而改变。相反，免疫后效应记忆（TEM） CD8+T细胞优先使用一个或多个TCR Vβ亚群。扩增的TCR Vβ在不同小鼠中表达频率不同，但Vβ4、6、7、8.3、9和11表达频率最高。此外，γ‐spz‐诱导的CD8+TEMin个体小鼠肝脏和血液中TCR Vβ的使用也存在相关性。血CD8+ tem1的扩增模式不随攻毒而改变，并在此后8周内保持不变。这些结果表明，γ - spz免疫会扭曲CD8+TEM的TCR Vβ库，并且长期维持特定的TCR Vβ表达。
Protection induced by irradiated Plasmodium berghei sporozoites (Pbγ‐spz) in mice is linked to CD8+T cells specific for exo‐erythrocytic‐stage Ags, and intrahepatic memory CD8+T cells are associated with protracted protection. However, the Ag specificity of the protective CD8+T cells remains largely unknown. In this study, we characterized the TCR Vβ usage by intrahepatic CD8+T cells during γ‐spz immunization and after the challenge with infectious Pb sporozoites. The repertoire of naïve (TN) and central memory (TCM) CD8+T cells was diverse and conserved between individual mice, and did not change with immunization. In contrast, preferential usage of one or more TCR Vβ subset was observed in effector memory (TEM) CD8+T cells after immunization. The expanded TCR Vβ varied between individual mice but Vβ4, 6, 7, 8.3, 9 and 11 were the most frequently expressed. In addition, there was a correlation in the TCR Vβ usage by γ‐spz‐induced CD8+TEMin the liver and blood of individual mice. The expansion pattern of blood CD8+TEMdid not change with challenge and remained the same for 8 weeks thereafter. These results demonstrate that immunization with γ‐spz skews the TCR Vβ repertoire of CD8+TEM, and commitment to a particular TCR Vβ expression is maintained long‐term.