Comparative study of susceptibilities of germinated and ungerminated conidia of Aspergillus fumigatus to various antifungal agents
Comparative study of susceptibilities of germinated and ungerminated conidia of Aspergillus fumigatus to various antifungal agents
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DOI：
10.1128/jcm.37.3.858-861.1999
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发表时间：
1999-03-01
影响因子：
9.4
通讯作者：
Chandrasekar, PH
中科院分区：
文献类型：
--
作者：
Manavathu, EK;Cutright, J;Chandrasekar, PH
分生孢子用作烟曲霉体外药敏试验的接种物。由于MIC是根据可见的菌丝生长来定义的，因此分生孢子应萌发并产生孢子（萌发的分生孢子），用于监测药物的生长抑制和杀菌活性。如果化合物能够抑制分生孢子的萌发，同时影响或不影响生物体的生长，则获得的MIC将是抑制分生孢子萌发所需的药物浓度，但不一定是抑制生物体生长所需的药物浓度。我们研究了萌发和未萌发分生孢子对两性霉素 B、伊曲康唑、伏立康唑和 SCH56592 的敏感性。各种抗真菌药物对萌发分生孢子的 MIC 与未萌发分生孢子的 MIC 几乎相同。此外，当暴露于药物24小时时，所有测试的化合物以几乎相同的效率杀死已发芽和未发芽的分生孢子。这些结果表明，已发芽或未发芽的分生孢子均可用作体外药敏研究的接种物。 A. fumigatus 具有相同的结果。
Conidia are used as inocula for the in vitro susceptibility testing of Aspergillus fumigauts. Since the MIC is defined on the basis of visible mycelial growth, conidia should germinate and produce sporelings (germinated conidia) for monitoring of the growth inhibition and fungicidal activity of a drug, If a compound is capable of inhibiting germination of conidia while affecting or not affecting the growth of the organism, the MIC obtained will be the concentration of the drug required for the inhibition of conidial germination but not necessarily that required for inhibition of the growth of the organism. We investigated the susceptibility of germinated and ungerminated conidia to amphotericin B, itraconazole, voriconazole, and SCH56592, The MICs of various antifungal agents for germinated conidia were almost identical to those obtained for ungerminated conidia. In addition, both the germinated and ungerminated conidia were killed with almost equal efficiency by all of the compounds tested when exposed to the drugs for 24 h, These results suggest that either germinated or ungerminated conidia could be used as inocula for in vitro susceptibility studies of. A. fumigatus with identical results.