Local and global dynamics of the basement membrane during branching morphogenesis require protease activity and actomyosin contractility
Local and global dynamics of the basement membrane during branching morphogenesis require protease activity and actomyosin contractility
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DOI：
10.1016/j.ydbio.2014.08.014
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发表时间：
2014-10-15
影响因子：
2.7
通讯作者：
Yamada, Kenneth M.
中科院分区：
文献类型：
--
作者：
Harunaga, Jill S.;Doyle, Andrew D.;Yamada, Kenneth M.
许多上皮组织在胚胎发育期间迅速扩张，同时保持被基底膜包围。基底膜的重塑被认为发生在分支形态发生期间以适应上皮生长，但这种重塑如何发生尚不清楚。我们报告说，基底膜是高度动态的分支过程中的唾液腺，表现出局部和全球的重塑。在上皮端芽的顶端，基底膜在快速扩张的区域被数百个定义明确的微观孔穿透。在局部，这导致可扩张的网状基底膜用于控制上皮。扩张的同时保持组织的完整性在全球范围内，基底膜作为一个整体向后移位，聚集在形成的次级导管周围，有助于在分支过程中稳定它们。基底膜的局部和全局动力学都需要蛋白酶和肌球蛋白II活性。我们的研究结果表明，基底膜是由蛋白水解降解，使其被移动和重塑细胞通过肌动球蛋白收缩，以支持分支形态发生。爱思唯尔公司出版
Many epithelial tissues expand rapidly during embryonic development while remaining surrounded by a basement membrane. Remodeling of the basement membrane is assumed to occur during branching morphogenesis to accommodate epithelial growth, but how such remodeling occurs is not yet clear. We report that the basement membrane is highly dynamic during branching of the salivary gland, exhibiting both local and global remodeling. At the tip of the epithelial end bud, the basement membrane becomes perforated by hundreds of well-defined microscopic holes at regions of rapid expansion. Locally, this results in a distensible, mesh-like basement membrane for controlled epithelial. expansion while maintaining tissue integrity Globally, the basement membrane translocates rearward as a whole, accumulating around the forming secondary ducts, helping to stabilize them during branching. Both local and global dynamics of the basement membrane require protease and myosin II activity. Our findings suggest that the basement membrane is rendered distensible by proteolytic degradation to allow it to be moved and remodeled by cells through actomyosin contractility to support branching morphogenesis. Published by Elsevier Inc.