Absorption and distribution of tea catechin, (-)-epigallocatechin-3-gallate, in the rat
Absorption and distribution of tea catechin, (-)-epigallocatechin-3-gallate, in the rat
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DOI：
10.3177/jnsv.43.679
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发表时间：
1997-12-01
影响因子：
1.6
通讯作者：
Miyazawa, T
中科院分区：
文献类型：
--
作者：
Nakagawa, K;Miyazawa, T
为研究抗癌茶儿茶素（-）-表没食子儿茶素没食子酸酯（EGCg）在大鼠体内的吸收和代谢，采用高效液相色谱法（CL-HPLC），测定了EGCg给药前后大鼠血浆、肝、脑、小肠粘膜和结肠粘膜中EGCg的浓度。用乙酸乙酯和甲醇连续提取，血浆中EGCg的回收率为86.1%，组织样品中EGCg的回收率为64.5-74.2%。对照组大鼠（给药前）血浆和组织样本中的EGCg浓度均低于检测限（
To investigate the absorption and metabolism of an anticarcinogenic tea catechin, (-)-epigallocatechin-3-gallate (EGCg), in rats, a newly developed chemiluminescence-detection high-performance liquid chromatography (CL-HPLC) method was employed and the EGCg concentrations in blood plasma, liver, brain, small intestinal mucosa and colon mucosa were determined before and after EGCg administration. The recovery of EGCg, extracted consecutively with ethyl acetate and methanol, was 86.1% from plasma and 64.5-74.2% from the tissue samples. The EGCg concentrations of plasma and tissue samples from the control rat (before EGCg administration) were all below the detection limit (