Association between two genetic variants of CD226 gene and Cervical Squamous Cell Carcinoma: a case-control study.
Association between two genetic variants of CD226 gene and Cervical Squamous Cell Carcinoma: a case-control study.
复制标题
DOI：
10.1016/j.gene.2012.11.039
复制
发表时间：
2013-04
期刊：
影响因子：
3.5
通讯作者：
Shaoqing Shi;Bin Zhou;Kui Zhang;Lin Zhang
中科院分区：
文献类型：
--
作者：
Shaoqing Shi;Bin Zhou;Kui Zhang;Lin Zhang
宫颈癌是常见的妇科肿瘤，严重影响全球女性的健康和生活质量。 CD226是一种共刺激分子，主要参与T细胞的活化和分化。最近的研究调查了CD226基因的两个遗传变异（rs763361和rs727088）与许多疾病之间的关联。为了评估这两种变异是否与宫颈鳞状细胞癌（CSCC）相关，我们进行了一项包括349名宫颈鳞状细胞癌患者和380名无关健康对照的病例对照研究，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和DNA测序方法确定这两种变异的基因型。观察到 CSCC 风险显着增加与 rs727088 位点的 G 等位基因相关（OR=1.422，95% CI=1.129–1.792）。我们还观察到，在显性模型中，CSCC 风险增加与 rs727088 多态性具有统计学相关性（OR=1.41，95% CI=1.05–1.89）。分层分析结果显示rs763361和rs727088多态性与临床特征无关。总的来说，这项研究支持 rs727088 多态性可能导致 CSCC 易感性增加。
Cervical carcinoma is a common gynecologic tumor severely influencing the health and life quality of women worldwide. CD226, a costimulatory molecule, is mainly participated in the activation and differentiation of T cells. Recent studies have investigated the association between two genetic variants (rs763361 and rs727088) of CD226 gene and many diseases. In order to evaluate whether these two variants are associated with Cervical Squamous Cell Carcinoma (CSCC), a case–control study including 349 CSCC patients and 380 unrelated healthy controls was carried out to determine the genotypes of these two variants by using the methods of polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and DNA sequencing methods. Significantly increased CSCC risk was observed to be associated with G allele of rs727088 locus (OR=1.422, 95% CI=1.129–1.792). We have also observed that increased CSCC risk was statistically associated with rs727088 polymorphism in a dominant model (OR=1.41, 95% CI=1.05–1.89). Results of stratified analysis revealed that both rs763361 and rs727088 polymorphisms were not associated with clinical characters. Collectively, this study supports that rs727088 polymorphism may contribute to increased CSCC susceptibility.