Increased Expression of miR-146a in Children With Allergic Rhinitis After Allergen-Specific Immunotherapy.
Increased Expression of miR-146a in Children With Allergic Rhinitis After Allergen-Specific Immunotherapy.
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DOI：
10.4168/aair.2016.8.2.132
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发表时间：
2016-03
期刊：
影响因子：
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通讯作者：
Li H
中科院分区：
文献类型：
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作者：
Luo X;Hong H;Tang J;Wu X;Lin Z;Ma R;Fan Y;Xu G;Liu D;Li H
MicroRNAs(MiRs)最近被认为对免疫细胞分化和免疫调节具有重要作用。然而，miRs是否参与过敏原特异性免疫治疗(SIT)在很大程度上仍不清楚。本研究旨在检测持续性变应性鼻炎(AR)儿童皮下免疫治疗(SCIT)和舌下免疫治疗(SIT)3个月后miR-146a和T调节细胞的变化。24例HDM致敏伴持续性变应性鼻炎的儿童接受SCIT(n=13)或Sit(n=11)治疗3个月。分别用定量逆转录聚合酶链式反应(qRT-PCR)、流式细胞仪和Western印迹分析检测治疗前后AR患者外周血单个核细胞(PBMC)miR-146a和Foxp3的相对表达、TRAF6蛋白水平和治疗后IL-10+CD4+T细胞与基础IL-10+CD4+T细胞的比值。采用双抗体夹心法测定血清IL-5、IL-10水平。治疗3个月后，TNSS和INSS评分均较治疗前显著降低(P<；0.01)。SCIT和SLIT后miR-146a和Foxp3mRNA的相对表达均显著增加(P<；0.01)。SCIT组和SLIT组治疗后IL-10+CD4+T细胞比例和血清IL-10水平均显著升高(P<；0.01)，而TRAF6蛋白水平和血清IL-5水平显著降低(P<；0.01)。在SCIT组和SILT组之间，这些生物标记物没有显著差异。我们的研究结果表明，miR-146a及其相关的生物标志物在SCIT和SIT后可能受到类似的调节，从而突出了miR-146a是改善AR管理的潜在治疗靶点。
MicroRNAs (miRs) were recently recognized to be important for immune cell differentiation and immune regulation. However, whether miRs were involved in allergen-specific immunotherapy (SIT) remains largely unknown. This study sought to examine changes in miR-146a and T regulatory cells in children with persistent allergic rhinitis (AR) after 3 months of subcutaneous immunotherapy (SCIT) and sublingual immunotherapy (SLIT). Twenty-four HDM-sensitized children with persistent AR were enrolled and treated with SCIT (n=13) or SLIT (n=11) for 3 months. Relative miR-146a and Foxp3 mRNA expression, the TRAF6 protein level, and the ratio of post-treatment to baseline IL-10+CD4+ T cells between the SCIT and SLIT groups were examined in the peripheral blood mononuclear cells (PBMCs) of AR patients using quantitative reverse transcription polymerase chain reaction (qRT-PCR), flow cytometry, and Western blot analysis, respectively. Serum levels of IL-5 and IL-10 were determined using ELISA. After 3 months of SIT, both the TNSS and INSS scores were significantly decreased compared to the baseline value (P<0.01). The relative expression of miR-146a and Foxp3 mRNA was significantly increased after both SCIT and SLIT (P<0.01). The ratio of post-treatment to baseline IL-10+CD4+ T cells and the serum IL-10 level were significantly increased in both the SCIT and SLIT groups (P<0.01), whereas the TRAF6 protein level and serum IL-5 level were significantly decreased (P<0.01). No significant differences in these biomarkers were observed between the SCIT and SLIT groups. Our findings suggest that miR-146a and its related biomarkers may be comparably modulated after both SCIT and SLIT, highlighting miR-146a as a potential therapeutic target for the improved management of AR.