Effects of MMP Inhibitors Incorporated within Dental Adhesives
Effects of MMP Inhibitors Incorporated within Dental Adhesives
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DOI：
10.1177/0022034512446339
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发表时间：
2012-06-01
影响因子：
7.6
通讯作者：
Banerjee, A.
中科院分区：
文献类型：
--
作者：
Almahdy, A.;Koller, G.;Banerjee, A.
基质金属蛋白酶（MMP）抑制剂已被证明可以减少粘合剂的降解，当作为预处理剂应用时，增加了粘合剂放置的临床步骤，但它们在牙科粘合剂中的结合尚未得到充分探索。本研究检查了在3种市售粘合剂的底漆中包含2种MMP抑制剂（BB94和GM6001）的效果。荧光测定和酶谱分析表明，与MMP抑制剂的粘合剂对合成的荧光FRET肽（95%）和牙本质粉末基板，分别有很高的亲和力。两种阻聚剂的加入均提高了两种胶粘剂的即时微拉伸粘接强度。然而，在储存3个月后，对照组和抑制剂组之间未检测到变化。改性的两步蚀刻和冲洗和单步系统显示出更少的罗丹明B渗透到“混合层”和“粘合剂”，分别。BB94和GM6001的掺入导致牙本质MMPs的抑制，提高了初始粘结强度和增强了封闭能力。
Matrix metalloproteinase (MMP) inhibition has been shown to reduce adhesive bond degradation when applied as a pre-conditioner, adding to clinical steps in the placement of adhesives, but their incorporation within dental adhesives has not been fully explored. This study examined the effect of including 2 MMP inhibitors (BB94 and GM6001) within the primers of 3 commercially available adhesives. Fluorometric assay and zymography showed that adhesives with MMP inhibitors had high affinity toward both synthetic fluorogenic FRET peptides (95%) and dentin powder substrates, respectively. The immediate microtensile bond strength was enhanced for 2 types of adhesives following the addition of both inhibitors. However, no changes were detected between the control and the inhibitor groups following 3-month storage. The modified two-step etch-and-rinse and single-step systems showed less Rhodamine B penetration to the "hybrid layer" and to the "adhesive", respectively. The incorporation of BB94 and GM6001 within the primers resulted in the inhibition of dentin MMPs with improved initial bond strength and enhanced sealing ability.