Human blood basophils produce interleukin-13 in response to IgE-receptor-dependent and -independent activation
Human blood basophils produce interleukin-13 in response to IgE-receptor-dependent and -independent activation
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DOI：
10.1182/blood.v88.8.3028.bloodjournal8883028
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发表时间：
1996-10-15
期刊：
影响因子：
20.3
通讯作者：
Dahinden, CA
中科院分区：
文献类型：
--
作者：
Ochensberger, B;Daepp, GC;Dahinden, CA
白细胞介素-13（IL-13）是新近发现的一种免疫调节细胞因子。IL-13的细胞来源及其表达的调控在很大程度上是未知的。在这里，我们表明，人嗜碱性粒细胞产生IL-13响应IgE受体（IgER）交联，IL-3，IL-3加C5 a，但不是单独的C5 a。人嗜碱性粒细胞以有限的方式表达IL-13，因为除了IL-4之外，细胞因子基因簇上编码的其他细胞因子（IL-3、IL-5和粒细胞巨噬细胞集落刺激因子[GM-CSF]）均未被诱导。最高水平的IL-13在IgE非依赖性活化后形成，导致IL-13的分泌延长。对IgER交联的应答更短暂，优先诱导IL-4。IL-3是调节人嗜碱性粒细胞产生IL-13的独特细胞因子：在大量测试的细胞因子中，仅IL-3能够直接诱导IL-13表达。此外，尽管在IL-5、GM-CSF、IGF-1或IL-1 β存在下响应于C5 a产生一些IL-13，但IL-3是迄今为止最有效的。放线菌素D和放线菌酮阻断IL-13的产生，导致IL-13释放的条件也导致IL-13 mRNA的诱导。这项研究支持人血嗜碱性粒细胞的重要免疫调节作用，由于他们的能力，同时表达IL-13和IL-4在一个有限的方式。(C)1996年，美国血液学会。
Interleukin-13 (IL-13) is a recently discovered immunoregulatory cytokine. The cellular sources of IL-13 and the regulation of its expression are largely unknown. Here we show that human basophils produce IL-13 in response to IgE-receptor (IgER) crosslinking, IL-3, IL-3 plus C5a, but not C5a alone. Human basophils express IL-13 in a restricted manner since, apart from IL-4, no other cytokines encoded on the cytokine gene cluster (IL-3, IL-5, and granulocyte macrophage-colony-stimulating factor [GM-CSF]), are induced. Highest levels of IL-13 are formed after IgE-independent activation leading to a prolonged secretion of IL-13. The response to IgER-crosslinking is more transient preferentially inducing IL-4. IL-3 is a unique cytokine regulating IL-13 production by human basophils: Among a large number of cytokines tested, only IL-3 is capable of directly inducing IL-13 expression. Furthermore, although some IL-13 is produced in response to C5a in the presence of IL-5, GM-CSF, IGF-1 or IL-1 beta, IL-3 is by far the most effective. IL-13 production was blocked by actinomycin D and cycloheximide and conditions leading to IL-13 release also lead to the induction of IL-13 mRNA. This study supports an important immunoregulatory role of human blood basophils, owing to their capacity to simultaneously express IL-13 and IL-4 in a restricted manner. (C) 1996 by The American Society of Hematology.