Time course of membrane microarchitecture-driven neovascularization.
Time course of membrane microarchitecture-driven neovascularization.
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膜微结构驱动的新血管形成的时间过程。
DOI：
10.1016/0142-9612(96)85565-7
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发表时间：
1996
期刊：
影响因子：
14
通讯作者：
Colton,CK
中科院分区：
文献类型：
--
作者：
Padera,RF;Colton,CK
宿主对诱导材料-组织界面处新生血管形成的微孔材料的反应根据侵入膜的细胞的数量和类型、材料-组织界面处的血管形成程度以及植入后作为时间函数的周围结缔组织的特性进行了研究。将标称孔径为8.0 μm的Millipore-MF混合酯纤维素膜皮下植入雄性Sprague-Dawley大鼠体内，并在植入后3、5、7、10、21和329天取出。在每次植入时，将两件器械的两个样本包埋在石蜡中，切片至5 μm厚，并用苏木精和伊红染色，进行光学显微镜观察。膜中的细胞密度在植入后增加至7天，然后在21天内保持大致恒定，并在329天时间点降低。材料-组织界面的血管分布增加至10天，甚至在植入后329天仍保持在该水平。结缔组织在第3天组织紊乱、疏松且无血管，在第5天类似于肉芽组织，并且在植入后第7天开始经历纤维囊形成和成熟。
The host response to a microporous material that induces neovascularization at the material-tissue interface was studied in terms of the number and types of cells invading the membrane, the degree of vascularization at the material-tissue interface, and the characteristics of the surrounding connective tissue as a function of time following implantation. Millipore-MF mixed esters of cellulose membranes with a nominal pore diameter of 8.0 μm were implanted subcutaneously into male Sprague-Dawley rats and explanted at 3, 5, 7, 10, 21 and 329 days post-implantation. Two samples from each of two devices at each implantation time were embedded in paraffin, sectioned to a thickness of 5 μm, and stained with haematoxylin and eosin for light microscopic observation. The density of cells in the membrane increased up to 7 days following implantation, then remained roughly constant through 21 days and decreased at the 329 day time point. The vascularity of the material-tissue interface increased up to 10 days and remained at this level even at 329 days post-implantation. The connective tissue was disorganized, loose and avascular at 3 days, resembled granulation tissue at 5 days, and underwent fibrous capsule formation and maturation starting at 7 days following implantation.