MONOCLONAL-ANTIBODIES AS PROBES OF THE ANTIGENIC STRUCTURE OF TOBACCO MOSAIC-VIRUS
MONOCLONAL-ANTIBODIES AS PROBES OF THE ANTIGENIC STRUCTURE OF TOBACCO MOSAIC-VIRUS
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DOI：
10.1002/j.1460-2075.1982.tb01285.x
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发表时间：
1982-01-01
期刊：
影响因子：
11.4
通讯作者：
VANREGENMORTEL, MHV
中科院分区：
文献类型：
--
作者：
ALMOUDALLAL, Z;BRIAND, JP;VANREGENMORTEL, MHV
通过测量9种单克隆抗体区分野生型病毒和13种在外壳蛋白中显示单和双氨基酸取代的突变体的能力，分析了烟草花叶病毒的抗原结构。尽管大多数抗体检测到位于病毒体外表面的那些取代，但其中一些抗体也识别由亚基内部深处发生的交换诱导的构象改变。在五种突变体的情况下，与野生型病毒相比，抗体反应性降低，而在另外三种突变体的情况下，抗体反应性显著更高。每种单克隆抗体对不同的取代具有独特的识别模式。两次交换的同时存在导致与九种抗体中的六种的任何结合完全消失，并且与另外三种抗体的结合减少。与多克隆抗血清相比，单克隆抗体的上级区分能力通过以下事实证明：当用单克隆抗体检测时，发现用抗血清未检测到的三次交换改变了抗原性。
The antigenic structure of tobacco mosaic virus has been analysed by measuring the ability of nine monoclonal antibodies to distinguish between wild‐type virus and 13 mutants showing single and double amino acid substitutions in the coat protein. Although the majority of antibodies detected those substitutions that were located at the outer surface of the virion, some of them also recognized conformation alterations induced by exchanges occurring deep inside the subunit. In the case of five mutants, the antibody reactivity was reduced compared with wild‐type virus, while in the case of three others, it was significantly higher. Each monoclonal antibody possessed a unique discrimination pattern with respect to the different substitutions. The simultaneous presence of two exchanges led to the complete disappearance of any binding with six of the nine antibodies and to reduced binding with three others. The superior discriminatory capacity of monoclonal antibodies compared with polyclonal antisera was demonstrated by the fact that three exchanges not detected with antisera were found to alter the antigenicity when tested with monoclonal antibodies.