miR-132 targeting cyclin E1 suppresses cell proliferation in osteosarcoma cells
miR-132 targeting cyclin E1 suppresses cell proliferation in osteosarcoma cells
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miR-132靶向细胞周期蛋白E1抑制骨肉瘤细胞增殖
DOI：
10.1007/s13277-014-1637-2
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发表时间：
2014-05-01
期刊：
影响因子：
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通讯作者：
Kang, Yifan
中科院分区：
文献类型：
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作者：
Wang, Jin;Xu, Guoxing;Kang, Yifan
在本研究中，我们研究了miR-132在骨肉瘤肿瘤生长中的作用。我们发现miR-132的过表达显著抑制体外细胞增殖和体内肿瘤生长。此外，miR-132过表达可诱导骨肉瘤细胞发生G1/S期阻滞。进一步研究发现，miR-132可与细胞周期蛋白E1（CCNE 1）基因的3′-非翻译区相互作用，抑制其表达。重新表达CCNE 1（不含3′UTR）可部分消除miR-132诱导的细胞增殖抑制。与CCNE 1相反，miR-132在骨肉瘤组织中的表达水平显著低于癌旁正常组织。综上所述，这些结果表明，miR-132在骨肉瘤中作为肿瘤抑制因子发挥作用，其抑制作用主要通过抑制CCNE 1表达介导。
In this study, we investigated the roles of miR-132 in tumor growth of osteosarcoma. We found that overexpression of miR-132 significantly suppressed in vitro cell proliferation and in vivo tumor growth. In addition, miR-132 overexpression induced G1/S cell cycle arrest of osteosarcoma cells. Further study showed that miR-132 could interact with the 3′-untranslated region of cyclin E1 (CCNE1) gene and repress its expression. Re-expression of CCNE1 (without the 3′UTR) could partially abrogate the miR-132-induced cell proliferation inhibition. Of significance, contrary to CCNE1, expression level of miR-132 was significantly lower in osteosarcoma tissues than in the adjacent normal tissues. Taken together, these results indicate that miR-132 functions as a tumor suppressor in osteosarcoma and that its suppressive effects are mediated chiefly by repressing CCNE1 expression.