Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography
Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography
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DOI：
10.1016/j.chroma.2006.07.044
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发表时间：
2006-09-15
影响因子：
4.1
通讯作者：
Xu, Hong Xi
中科院分区：
文献类型：
--
作者：
Bin Han, Quan;Song, Jing Zheng;Xu, Hong Xi
首次采用高速逆流色谱仪与柱切换阀联用，建立了循环逆流色谱系统。该体系首次成功地应用于藤黄中差向异构体藤黄酸和表藤黄酸的制备性分离，采用正己烷-甲醇-水（5：4：1，v/v/v）为两相溶剂体系。结果，从50 mg混合物中分离出28.2 mg藤黄酸和18.4 mg表藤黄酸。经高效液相色谱法测定，其纯度均在97%以上。然后通过它们的H-1 NMR和C-13 NMR谱鉴定化学结构。(c)2006 Elsevier B. V.保留所有权利。
A recycling counter-current chromatographic system was first set up with a high-speed counter-current chromatography instrument coupled with a column switching valve. This system was first successfully applied to the preparative separation of epimers, gambogic acid and epigambogic acid from Garcinia hanburyi using n-hexane-methanol-water (5:4: 1, v/v/v) as the two-phase solvent system. As a result, 28.2 mg gambogic acid and 18.4 mg epigambogic acid were separated from 50 mg of mixture. Their purities were both above 97% as determined by HPLC. The chemical structures were then identified by their H-1 NMR and C-13 NMR spectra. (c) 2006 Elsevier B.V. All rights reserved.