A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov.
A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov.
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DOI：
10.1139/m78-160
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发表时间：
1978
影响因子：
2.8
通讯作者：
J. Tarrand;N. Krieg;J. Döbereiner
中科院分区：
文献类型：
--
作者：
J. Tarrand;N. Krieg;J. Döbereiner
在蛋白胨-琥珀酸盐培养基中，61株根相关固氮菌Spirrillum lipoferum表现出相似的形态：直径为1.0 μ m的类弧菌细胞。当在肉汤中生长时，细胞具有单个极鞭毛，但是当在30 ° C的琼脂上生长时，也形成较短波长的侧鞭毛。热变性法测得DNA碱基组成为69 - 71mol%鸟嘌呤+胞嘧啶。DNA同源性实验表明，发生两个不同的，但相关的同源性组：46株在组I和15株在组II。组II菌株的区别在于它们能够使用葡萄糖作为唯一的碳源在无氮培养基中生长，通过它们在基于蛋白胨的葡萄糖培养基中产生酸性反应，通过它们对生物素的需求，以及通过它们在半固体无氮苹果酸盐培养基中形成更宽、更长、S形或螺旋形细胞。结果表明，存在两个种，并根据其特征，建议将它们分配到一个新属，Azoacellum。属于第二组的Strians被命名为A。Lipoferum（Beijerinck）comb. nov.，而属于组I的那些被命名为A。菌株Sp 59b（ATCC 29707）为该菌的新模式菌株。sp7（ATCC 29145）为模式菌株。巴西语。
Sixty-one strains of the root-associated nitrogen fixer Spirillum lipoferum exhibited a similar morphology in peptone--succinate salts medium: vibrioid cells having a diameter of 1.0 micrometer. When grown in broth the cells had a single polar flagellum, but when grown on agar at 30 degrees C lateral flagella of shorter wavelength were also formed. The DNA base composition was 69--71 mol% guanine + cytosine when determined by thermal denaturation. DNA homology experiments indicated the occurrence of two distinct but related homology groups: 46 strains were in group I and 15 strains were in group II. Group II strains were distinguished by their ability to use glucose as a sole carbon source for growth in nitrogen-free medium, by their production of an acidic reaction in a peptone-based glucose medium, by their requirement for biotin, and by their formation of wider, longer, S-shaped or helical cells in semisolid nitrogen-free malate medium. The results indicate that two species exist, and on the basis of their characteristics it is proposed that they be assigned to a new genus, Azospirillum. Strians belonging to group II are named A. lipoferum (Beijerinck) comb. nov., while those belonging to group I are named A. brasilense sp. nov. Strain Sp 59b (ATCC29707) is proposed as the neotype strain for A. lipoferum, and strain Sp 7 (ATCC 29145) is proposed as the type strain for A. brasilense.