Local and global consequences of flow on bacterial quorum sensing.
Local and global consequences of flow on bacterial quorum sensing.
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DOI：
10.1038/nmicrobiol.2015.5
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发表时间：
2016-01-11
影响因子：
28.3
通讯作者：
Stone, Howard A.
中科院分区：
文献类型：
--
作者：
Kim, Minyoung Kevin;Ingremeau, Francois;Zhao, Aishan;Bassler, Bonnie L.;Stone, Howard A.
细菌使用一种称为群体感应（QS）的化学通讯过程来控制集体行为，如发病机制和生物膜的形成。QS依赖于被称为自诱导剂的信号分子的产生、释放和群体范围内的检测。迄今为止，在混合良好的培养物中对细菌发病机制的研究已经揭示了毒力因子和控制它们的调控回路，包括QS的首要作用。虽然流动在几乎所有的生命系统中都是普遍存在的，但很少有人探索QS是如何在模拟宿主环境的情况下影响致病性状的，例如，在流体流动和复杂的几何形状下。先前的研究表明，足够强的流动抑制了QS。然而，目前尚不清楚QS在恒定或间歇流动下如何发挥作用，它在生物膜内如何变化，或作为受限流动位置的函数，或表面形貌（沟槽、裂缝、孔隙）如何影响QS介导的通讯。我们用两种常见的病原体金黄色葡萄球菌和霍乱弧菌来探讨这些问题。我们确定了流动抑制QS的条件以及尽管流动仍激活QS的其他条件，包括表征影响QS响应的几何和地形特征。我们的研究强调，在流动下，基因相同的细胞在空间和时间上不表现出与QS相关的表型一致性，导致复杂的发病和定植模式。了解现实环境中时空不均匀QS响应的后果对于成功部署综合的支持和反对QS策略至关重要。
Bacteria use a chemical communication process called quorum sensing (QS) to control collective behaviours, such as pathogenesis and biofilm formation. QS relies on the production, release, and group-wide detection of signal molecules called autoinducers. To date, studies of bacterial pathogenesis in well-mixed cultures have revealed virulence factors and the regulatory circuits controlling them, including the overarching role of QS. Although flow is ubiquitous to nearly all living systems, much less explored is how QS influences pathogenic traits in scenarios that mimic host environments, for example, under fluid flow and in complex geometries. Previous studies have showed that sufficiently strong flow represses QS. Nonetheless, it is not known how QS functions under constant or intermittent flow, how it varies within biofilms or as a function of position along a confined flow, or how surface topography (grooves, crevices, pores) influence QS-mediated communication. We explore these questions using two common pathogens Staphylococcus aureus and Vibrio cholerae. We identify conditions where flow represses QS and other conditions where QS is activated despite flow, including characterizing geometric and topographic features that influence the QS response. Our studies highlight that, under flow, genetically identical cells do not exhibit phenotypic uniformity with respect to QS in space and time, leading to complex patterns of pathogenesis and colonization. Understanding the ramifications of spatially and temporally non-uniform QS responses in realistic environments will be crucial for successful deployment of synthetic pro- and anti-QS strategies.
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DOI：
10.1073/pnas.1300321110
发表时间：
2013-03-12
影响因子：
11.1
作者：
Drescher, Knut;Shen, Yi;Stone, Howard A.
通讯作者：
Stone, Howard A.
影响因子：
3.2
作者：
Kirisits, Mary Jo;Margolis, Jeffrey J.;Parsek, Matthew R.
通讯作者：
Parsek, Matthew R.
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
2
作者：
Meyer, Andrea;Megerle, Judith A.;Raedler, Joachim O.
通讯作者：
Raedler, Joachim O.
影响因子：
64.5
作者：
Persat A;Nadell CD;Kim MK;Ingremeau F;Siryaporn A;Drescher K;Wingreen NS;Bassler BL;Gitai Z;Stone HA
通讯作者：
Stone HA