Dissemination of microbiota between wounds and the beds of patients with pressure injuries: a cross-sectional study
Dissemination of microbiota between wounds and the beds of patients with pressure injuries: a cross-sectional study
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DOI：
10.33235/wpr.29.2.70-76
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发表时间：
2021-06-01
影响因子：
0.6
通讯作者：
Sanada, H.
中科院分区：
文献类型：
--
作者：
Kunimitsu, M.;Nakagami, G.;Sanada, H.
目的伤口感染是压迫性损伤（Pis）的一种危及生命的并发症，目前还不能完全预防。本研究旨在探讨微生物群之间的传播Pls和医院病床使用培养独立的方法。这是开发预防伤口感染的新干预措施的第一步。方法对某长期护理医院年龄为bb0 = 65岁的Pls患者进行横断面研究，采用16S核糖体RNA （rRNA）基因测序分析伤口、皮肤和床上的微生物群。采用零半径操作分类单位（Zero-radius operational taxonomic units, zOTUs）进行传播鉴定，表明细菌在16S rRNA基因V3-V4区具有相同的序列。结果本研究分析了10个Pls。所有个体的伤口、皮肤和床上样本均有相同的zOTUs（中位数：194，四分位数间距[IQR]: 121-320）。此外，8个地点的床上样本与伤口样本被归为相同的聚类。我们的研究是第一个使用培养独立分析定量显示微生物群在Pls和患者床之间传播的研究。防止细菌传播到床上可能是预防伤口感染的有效治疗策略。
Aim Wound infection is a life-threatening complication of pressure injuries (Pis) and is not yet completely preventable. This study aims to explore the dissemination of microbiota between Pls and hospital beds using a culture-independent methodology. This serves as the first step towards developing a new intervention to prevent wound infection.Methods A cross-sectional study was conducted at a long-term care hospital on patients aged >= 65 years with Pls. The microbiota of wounds, skin and beds were identified using 16S ribosomal RNA (rRNA) gene sequencing analysis. Zero-radius operational taxonomic units (zOTUs) were used for confirming dissemination which indicates bacteria possessing identical sequences within the V3-V4 region of the 16S rRNA gene.Results Ten Pls were analysed in this study. All individuals had zOTUs common to samples from their wound, skin and bed (median: 194, interquartile range [IQR]: 121-320). Furthermore, the bed samples were classified into the same clusters as the wound samples from eight sites.Conclusion Our study is the first to quantitatively show the dissemination of microbiota between Pls and patients' beds using culture-independent analysis. Preventing the dissemination of bacteria to beds may be an effective therapeutic strategy for the prevention of wound infection.