Multiple latent viruses reactivate in astronauts during Space Shuttle missions
Multiple latent viruses reactivate in astronauts during Space Shuttle missions
复制标题
DOI：
10.1016/j.bbi.2014.05.014
复制
发表时间：
2014-10-01
影响因子：
15.1
通讯作者：
Pierson, D. L.
中科院分区：
文献类型：
--
作者：
Mehta, S. K.;Laudenslager, M. L.;Pierson, D. L.
对17名航天员(男16名，女1名)在短时间(12-16天)航天飞机飞行前、中、后的潜伏病毒复活及昼夜唾液皮质醇和脱氢表雄酮进行了前瞻性测定。在每个阶段都采集了血液、尿液和唾液样本。检测EB病毒(EBV)、水痘-带状疱疹病毒(VZV)和巨细胞病毒(CMV)的抗病毒抗体和病毒载量(DNA)。三名宇航员在飞行前、飞行中或飞行后采集的任何样本中都没有携带任何病毒。在所有三个飞行阶段，其余14名宇航员的唾液中都有EB病毒。14名EB病毒携带者中有7人在飞行期间和之后的唾液样本中也检出了VZV，14名EBV携带者中有8人在飞行前、期间和之后的尿样中也检出了CMV。在14名机组人员中的6名中，所有3种目标病毒都在一个或多个飞行阶段中被释放。EBV和VZV DNA拷贝在飞行阶段均高于飞行前或飞行后水平。与飞行后相比，飞行前外周血中的EBV DNA有所增加。18名健康对照也包括在研究中。大约2%-5%的对照组感染了EBV，而没有人感染VZV或CMV。飞行前和飞行中测得的唾液皮质醇均较飞行后升高。相比之下，DHEA在飞行阶段相对于飞行前和飞行后都有所下降。因此，在飞行过程中，皮质醇/脱氢表雄酮日曲线下面积相对于地面的摩尔比(AUCG)显著增加。这一比例与病毒脱落无关。总而言之，在航天飞行期间，三种疱疹病毒可以单独或联合重新激活。(C)2014 Elsevier Inc.保留所有权利。
Latent virus reactivation and diurnal salivary cortisol and dehydroepiandrosterone were measured prospectively in 17 astronauts (16 male and 1 female) before, during, and after short-duration (12-16 days) Space Shuttle missions. Blood, urine, and saliva samples were collected during each of these phases. Antiviral antibodies and viral load (DNA) were measured for Epstein-Barr virus (EBV), varicella-zoster virus (VZV), and cytomegalovirus (CMV). Three astronauts did not shed any virus in any of their samples collected before, during, or after flight. EBV was shed in the saliva in all of the remaining 14 astronauts during all 3 phases of flight. Seven of the 14 EBV-shedding subjects also shed VZV during and after the flight in their saliva samples, and 8 of 14 EBV-shedders also shed CMV in their urine samples before, during, and after flight. In 6 of 14 crewmembers, all 3 target viruses were shed during one or more flight phases. Both EBV and VZV DNA copies were elevated during the flight phase relative to preflight or post-flight levels. EBV DNA in peripheral blood was increased preflight relative to post-flight. Eighteen healthy controls were also included in the study. Approximately 2-5% of controls shed EBV while none shed VZV or CMV. Salivary cortisol measured preflight and during flight were elevated relative to post-flight. In contrast DHEA decreased during the flight phase relative to both preflight and post-flight. As a consequence, the molar ratio of the area under the diurnal curve of cortisol to DHEA with respect to ground (AUCg) increased significantly during flight. This ratio was unrelated to viral shedding. In summary, three herpes viruses can reactivate individually or in combination during spaceflight. (C) 2014 Elsevier Inc. All rights reserved.