Effect of a purified atrial natriuretic factor on rat and rabbit vascular strips and vascular beds.
Effect of a purified atrial natriuretic factor on rat and rabbit vascular strips and vascular beds.
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纯化的心房钠尿因子对大鼠和兔血管条和血管床的影响。
DOI：
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发表时间：
1984
影响因子：
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通讯作者：
J. Genest
中科院分区：
文献类型：
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作者：
R. Garcia;G. Thibault;M. Cantin;J. Genest
大鼠心房肌细胞内含有一种功能强大的利钠利尿肽，这种肽定位于特定的颗粒中。这种心房利钠因子（ANF）产生了一个强大的，剂量依赖性的舒张作用，对兔和大鼠动脉条先前作出的合同，无论是应用去甲肾上腺素（NE）或血管紧张素II。如果KCl用作收缩剂或在任何条件下使用兔肠系膜条，均未观察到该效应。应用ANF后，血管条对NE或血管紧张素II的后续刺激不敏感。心钠素输注到一个高阻力的隔离灌注大鼠肾脏产生了快速下降（33 +/- 5毫米汞柱）的灌注压，持续18 +/- 3分钟。这种效果是没有看到在隔离大鼠肠系膜动脉制备，即使当灌注压升高的NE输注。ANF对血管平滑肌的这些作用不是由肾上腺素、α-和β-肾上腺素能受体和毒蕈碱受体介导的，也不是由Ca 2+内流的损伤介导的，但它们被硝普钠模拟。低分子量和高分子量ANF产生相同的效果。这些肽的特定的受体位点的存在建议。
Rat atrium cardiocytes contain a powerful natriuretic and diuretic peptide that has been localized in the specific granules. This atrial natriuretic factor (ANF) produced a potent, dose-dependent relaxant effect on rabbit and rat arterial strips previously made to contract by application of either norepinephrine (NE) or angiotensin II. The effect was not seen if KCl was used as contractile agent or under any conditions with rabbit mesenteric strips. After the application of ANF the vascular strips were refractory to subsequent stimulation by either NE or angiotensin II. The infusion of ANF into a high-resistance isolated perfused rat kidney produced a rapid decrease (33 +/- 5 mmHg) in perfusion pressure that lasted for 18 +/- 3 min. This effect was not seen in the isolated rat mesenteric arterial preparation, even when the perfusion pressure was raised by the infusion of NE. These effects of ANF on vascular smooth muscle are not mediated by prostaglandins, by alpha- and beta-adrenergic and muscarinic receptors, or by an impairment of Ca2+ influx, but they are mimicked by sodium nitroprusside. A low- and a high-molecular-weight ANF produced the same effects. The existence of specific receptive sites for these peptides is suggested.