Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells.
Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells.
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DOI：
10.17912/micropub.biology.000716
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发表时间：
2022
影响因子：
--
通讯作者：
Kemp, Michael G
中科院分区：
文献类型：
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作者：
Gaikwad, Prashant;Kemp, Michael G
一些研究表明，在不同的细胞条件下，包括分化、衰老和静止期间，组织蛋白酶L （CTSL）蛋白水解活性在细胞核中发挥作用。在这里，我们展示了在细胞裂解缓冲液中添加CTSL抑制剂可以阻止静止人类细胞裂解过程中几种核蛋白的裂解，包括以前认为在其他细胞和组织环境中具有功能相关性的蛋白质。这些发现表明，在裂解含有高水平CTSL蛋白的细胞和组织时，应谨慎使用CTSL抑制剂，以区分体内和体外人工发生的蛋白质水解。
Several studies have indicated a role for cathepsin L (CTSL) proteolytic activity in the nucleus under distinct cellular conditions, including during differentiation, senescence, and quiescence. Here we show that addition of CTSL inhibitors to a cell lysis buffer prevents the cleavage of several nuclear proteins during the lysis of quiescent human cells, including proteins previously thought to have functional relevance in other cell and tissue contexts. These findings suggest that care should be taken to use CTSL inhibitors when lysing cells and tissues containing high levels of CTSL protein to differentiate proteolysis that occurs in vivo versus artifactually in vitro.