Hypoxic X-irradiation as an external stimulus for conformational change of oligodeoxynucleotides that possess disulfide bond and regulation of DNAzyme function.
Hypoxic X-irradiation as an external stimulus for conformational change of oligodeoxynucleotides that possess disulfide bond and regulation of DNAzyme function.
复制标题
低氧 X 射线作为外部刺激，使具有二硫键的寡脱氧核苷酸发生构象变化并调节 DNAzyme 功能。
DOI：
10.1016/j.bmcl.2014.11.043
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Tanabe;K.; Okada;K.; Sugiura;M.; Ito;T.; Nishimoto;S.
我们利用二硫键的辐解链交换反应实现了寡脱氧核苷酸的构象变化和DNA酶功能的调控。我们设计了一个系统，在该系统中，通过具有二硫键的抑制剂链与活性DNAzyme的杂交来抑制DNAzyme切割RNA的功能。低剂量X射线照射导致RNA切割的恢复，因为抑制剂链中二硫键处的链交换反应导致抑制剂链的释放。这一策略可能适用于低氧X射线照射的基因调控。
We achieved a conformational change of oligodeoxynucleotides and the regulation of DNAzyme function by means of a radiolytic strand exchange reaction of disulfide bond. We designed a system in which the DNAzyme function of RNA cleavage was suppressed by the hybridization of an inhibitor strand that possessed disulfide bond with an active DNAzyme. Hypoxic X-irradiation led to the recovery of RNA cleavage because the strand exchange reaction at the disulfide bond in inhibitor strand resulted in a release of inhibitor strand. This strategy may be applicable to gene regulation by hypoxic X-irradiation.