Expression of Cbl-interacting protein of 85 kDa in MPTP mouse model of Parkinson's disease and 1-methyl-4-phenyl-pyridinium ion-treated dopaminergic SH-SY5Y cells
Expression of Cbl-interacting protein of 85 kDa in MPTP mouse model of Parkinson's disease and 1-methyl-4-phenyl-pyridinium ion-treated dopaminergic SH-SY5Y cells
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帕金森病 MPTP 小鼠模型和 1-甲基-4-苯基-吡啶鎓离子处理的多巴胺能 SH-SY5Y 细胞中 85 kDa Cbl 相互作用蛋白的表达
DOI：
10.1111/j.1745-7270.2008.00423.x
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发表时间：
2008-06-01
影响因子：
3.7
通讯作者：
Huang, Fang
中科院分区：
文献类型：
--
作者：
Bian, Minjuan;Yu, Mei;Huang, Fang
新发现的85 kDa的Cbl相互作用蛋白（CIN 85）参与许多细胞过程，但其在脑和神经退行性疾病中的功能仍不清楚。本研究通过Western blot和免疫组织化学方法研究了CIN 85蛋白在成年小鼠脑内不同区域的分布，发现CIN 85在小鼠脑内广泛表达。在纹状体和黑质这两个帕金森病发病最深的区域，CIN 85蛋白水平相对较高。在MPTP帕金森病小鼠模型中，CIN 85在纹状体和黑质的表达是复杂的。而在1-甲基-4-苯基吡啶离子处理的SH-SY 5 Y细胞中，CIN 85的表达显著增加。通过短发夹RNA敲低CIN 85减少SH-SY 5 Y细胞死亡。因此，CIN 85可能在多巴胺能细胞系和神经毒素攻击下的小鼠脑黑质纹状体中发挥不同的作用。
The newly discovered Cbl-interacting protein of 85 kDa (CIN85) is involved in many cellular processes, but its functions in the brain and in neurodegenerative diseases remain unclear. In this paper, we investigated the distribution of CIN85 protein in different regions of adult mouse brain using Western blot analysis and immunohistochemistry, and found that CIN85 was ubiquitously expressed in mouse brain. In the striatum and substantia nigra, two regions most deeply affected in Parkinson's disease, the level of CIN85 protein was relatively high. In the MPTP mouse model of Parkinson's disease, the expression of CIN85 in the striatum and substantia nigra was complicated. But in 1-methyl-4-phenyl-pyridinium ion-treated human dopaminergic SH-SY5Y cells, the expression of CIN85 increased dramatically. Knocking down of CIN85 by short hairpin RNA reduced SH-SY5Y cell death. Therefore, CIN85 might play different roles in the dopaminergic cell line and in the nigrostriatum of mouse brain under neurotoxin challenge.