Genetic mapping and phenotypic analysis of shot(H.3.2) in Drosophila melanogaster.
Genetic mapping and phenotypic analysis of shot(H.3.2) in Drosophila melanogaster.
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DOI：
10.17912/micropub.biology.000418
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发表时间：
2021
影响因子：
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通讯作者：
Merkle JA
中科院分区：
文献类型：
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作者：
Talley EM;Watts CT;Aboyer S;Adamson MG;Akoto HA;Altemus H;Avella PJ;Bailey R;Bell ER;Bell KL;Breneman K;Burkhart JS;Chanley LJ;Cook SS;DesLaurier MT;Dorsey TR;Doyle CJ;Egloff ME;Fasawe AS;Garcia KK;Graves NP;Gray TK;Gustafson EM;Hall MJ;Hayes JD;Holic LJ;Jarvis BA;Klos PS;Kritzmire S;Kuzovko L;Lainez E;McCoy S;Mierendorf JC;Neri NA;Neville CR;Osborn K;Parker K;Parks ME;Peck K;Pitt R;Platta ME;Powell B;Rodriguez K;Ruiz C;Schaefer MN;Shields AB;Smiley JB;Stauffer B;Straub D;Sweeney JL;Termine KM;Thomas B;Toth SD;Veile TR;Walker KS;Webster PN;Woodard BJ;Yoder QL;Young MK;Zeedyk ML;Ziegler LN;Bieser KL;Puthoff DP;Stamm J;Vrailas-Mortimer AD;Kagey JD;Merkle JA
遗传筛选用于识别参与特定生物过程的基因。果蝇的 EMS 诱变筛选确定了发育中眼睛的生长控制表型。该筛选中的一个突变系 H.3.2 使用 FLP/FRT 系统进行了表型表征，并由参与多机构 Fly-CURE 联盟的本科生通过互补分析和基因组测序进行了遗传图谱分析。发现 H.3.2 在哺乳动物光谱肌张力障碍蛋白 (DST) 的同源物短期停止 (shot) 中具有无义突变。 shot 和 DST 参与细胞骨架组织，并在细胞生长和增殖过程中发挥作用。
Genetic screens are used to identify genes involved in specific biological processes. An EMS mutagenesis screen in Drosophila melanogaster identified growth control phenotypes in the developing eye. One mutant line from this screen, H.3.2, was phenotypically characterized using the FLP/FRT system and genetically mapped by complementation analysis and genomic sequencing by undergraduate students participating in the multi-institution Fly-CURE consortium. H.3.2 was found to have a nonsense mutation in short stop (shot), anortholog of the mammalian spectraplakin dystonin (DST). shot and DST are involved in cytoskeletal organization and play roles during cell growth and proliferation.