HPLC determination of enoxacin, ciprofloxacin, norfloxacin and ofloxacin with photoinduced fluorimetric (PIF) detection and multiemission scanning -: Application to urine and serum
HPLC determination of enoxacin, ciprofloxacin, norfloxacin and ofloxacin with photoinduced fluorimetric (PIF) detection and multiemission scanning -: Application to urine and serum
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DOI：
10.1016/j.jchromb.2005.05.045
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发表时间：
2005-08-05
影响因子：
3
通讯作者：
Salinas, F
中科院分区：
文献类型：
--
作者：
Espinosa-Mansilla, A;de la Peña, AM;Salinas, F
在乙醇-水介质中，氟喹诺酮类药物依诺沙星 (ENO)、环丙沙星 (CIPRO)、诺氟沙星 (NOR) 和氧氟沙星 (OFLO) 的荧光发射在几分钟内通过紫外线照射显着增加。已经开发出一种基于所形成的辐照光产物的分离的 HPLC 方法，用于测定这些氟喹诺酮类药物。通过快速多重扫描荧光检测对分析波长进行了优化。 ENO 和 OFLO 的发射波长分别为 407 和 490 nm，NOR 和 CIPRO 的发射波长为 444 nm（激发波长为 277 nm），灵敏度最高。根据 Clayton 标准，使用 0.05 作为假阳性和假阴性误差保证概率，发现 ENO、NOR、CIPRO 和 OFLO 的检测限分别为 7.3、6.0、6.3 和 14.5 ng/mL。尿液和血清样本已成功分析，尿液和血清的回收率分别为 99-97% 和 98-103%。 (c) 2005 Elsevier B.V. 保留所有权利。
The fluorescence emission of the fluoroquinolones enoxacin (ENO), ciprofloxacin (CIPRO), norfloxacin (NOR) and ofloxacin (OFLO) notably increased by UV irradiation during few minutes, in ethanolic-water medium. An HPLC method has been developed, for the determination of these fluoroquinolones, based in the separation of the formed irradiation photoproducts. Optimization of the analytical wavelengths has been carried out by fast multiernission scanning fluorescence detection. The highest sensitivity has been found when measuring at emission wavelengths of 407 and 490 nm, for ENO and OFLO, respectively, and at 444 nm for both NOR and CIPRO (exciting at 277 nm). According to the criterium of Clayton, using 0.05 as false positive and false negative error assurance probabilities, detection limits of 7.3, 6.0, 6.3 and 14.5 ng/mL, for ENO, NOR, CIPRO and OFLO, respectively, have been found. Urine and serum samples have been successfully analyzed, with recovery values ranging among 99-97% and 98-103%, for urine and serum, respectively. (c) 2005 Elsevier B.V. All rights reserved.