Selective Killing Effects of Cold Atmospheric Pressure Plasma with NO Induced Dysfunction of Epidermal Growth Factor Receptor in Oral Squamous Cell Carcinoma.
Selective Killing Effects of Cold Atmospheric Pressure Plasma with NO Induced Dysfunction of Epidermal Growth Factor Receptor in Oral Squamous Cell Carcinoma.
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DOI：
10.1371/journal.pone.0150279
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Kim KN
中科院分区：
文献类型：
--
作者：
Lee JH;Om JY;Kim YH;Kim KM;Choi EH;Kim KN
本研究的目的是探讨冷大气压等离子体（CAP）诱导的自由基对表皮生长因子受体（EGFR）的影响，这是过度表达的口腔鳞状细胞癌，以确定潜在的机制选择性杀伤。CAP诱导的高活性自由基，观察到在血浆羽和细胞培养基。以正常人牙龈成纤维细胞为对照，观察其对口腔鳞癌细胞的选择性杀伤作用。EGFR的降解和功能障碍只在EGFR过表达的口腔鳞状细胞癌中观察到，而不是在正常细胞中。CAP治疗前在细胞培养基中预处理一氧化氮清除剂可挽救上述EGFR的降解和功能障碍以及对口腔鳞状细胞癌的杀伤作用。CAP通过一氧化氮自由基诱导EGFR过度表达的口腔鳞状细胞癌中EGFR功能障碍，可能是一种有前途的癌症治疗方法。
The aim of this study is to investigate the effects of cold atmospheric pressure plasma (CAP)-induced radicals on the epidermal growth factor receptor (EGFR), which is overexpressed by oral squamous cell carcinoma, to determine the underlying mechanism of selective killing. CAP-induced highly reactive radicals were observed in both plasma plume and cell culture media. The selective killing effect was observed in oral squamous cell carcinoma compared with normal human gingival fibroblast. Degradation and dysfunction of EGFRs were observed only in the EGFR-overexpressing oral squamous cell carcinoma and not in the normal cell. Nitric oxide scavenger pretreatment in cell culture media before CAP treatment rescued above degradation and dysfunction of the EGFR as well as the killing effect in oral squamous cell carcinoma. CAP may be a promising cancer treatment method by inducing EGFR dysfunction in EGFR-overexpressing oral squamous cell carcinoma via nitric oxide radicals.