Early induction of moderate hypothermia suppresses systemic inflammatory cytokines and intracellular adhesion molecule-1 in rats with caerulein-induced pancreatitis and endotoxemia
Early induction of moderate hypothermia suppresses systemic inflammatory cytokines and intracellular adhesion molecule-1 in rats with caerulein-induced pancreatitis and endotoxemia
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DOI：
10.1097/mpa.0b013e318162cb26
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发表时间：
2008-08-01
期刊：
影响因子：
2.9
通讯作者：
Maekawa, Tsuyoshi
中科院分区：
文献类型：
--
作者：
Fujimoto, Kenji;Fujita, Motoki;Maekawa, Tsuyoshi
目的：探讨亚低温（MH）对大鼠急性胰腺炎（AP）和内毒素血症的全身影响及诱导时机。比较4组（假手术组，Sham组）的作用（38 ℃），对照组（38 ℃）、早期MH组（32 ℃，给予脂多糖[LPS]）和延迟MH组（32 ℃，LPS后1小时）。结果：MH组和MH组大鼠血清IL-6水平均显著低于对照组（P < 0. 05），MH组大鼠血清IL-6水平显著低于MH组（P < 0. 05）。早期MH组血清IL-10水平在1h时明显高于其他3组。MH组IL-10/IL-6比值在3 h时显著高于对照组。血清可溶性细胞间粘附分子（sICAM-1）在MH组和对照组均显著低于3小时。早期MH组血清sICAM-1水平明显低于延迟MH组。胰腺ICAM-1的变化趋势与血清sICAM-1的变化趋势相似。结论：早期诱导MH对AP和内毒素血症时胰腺损伤及全身炎症反应具有保护作用。
Objectives: To evaluate the systemic effects of moderate hypothermia (MH) and the timing of induction on acute pancreatitis (AP) and endotoxemia in rats.Methods: The effects of MH were compared in 4 groups, that is, sham group (38 degrees C), control group (38 degrees C), early MH group (32 degrees C on administration of lipopolysaccharide [LPS]), and delayed MH group (32 degrees C 1 hour after LPS). AP and endotoxemia were induced by intramuscular injection of caerulein and intraperitoneal injection of LPS.Results: Serum interleukin 6 (IL-6) in both MH groups was significantly lower than that in the control group at 3 hours. Serum interleukin 10 (IL-10) in the early MH group was significantly higher than those in the other 3 groups at 1 hour. IL-10/IL-6 ratios in both MH groups were significantly higher than that in the control group at 3 hours. Serum soluble intercellular adhesion molecule (sICAM-1) in both MH groups was significantly lower than that in the control group at 3 hours. Serum sICAM-1 in the early MH group was significantly lower than that in the delayed MH group. The tendency of pancreatic ICAM-1 was similar to that of serum sICAM-1.Conclusions: Early induction of MH might be protective against pancreatic injury and systemic inflammation in AP and endotoxemia.