Population Structure and Phylogenetic Characterization of Marine Benthic Archaea in Deep-Sea Sediments
Population Structure and Phylogenetic Characterization of Marine Benthic Archaea in Deep-Sea Sediments
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DOI：
10.1128/aem.65.10.4375-4384.1999
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发表时间：
1999-10
影响因子：
4.4
通讯作者：
C. Vetriani;H. Jannasch;B. MacGregor;D. Stahl;A. Reysenbach
中科院分区：
文献类型：
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作者：
C. Vetriani;H. Jannasch;B. MacGregor;D. Stahl;A. Reysenbach
摘要在过去的几年里，古细菌被认为是海洋微型浮游生物组合的一个广泛而重要的组成部分，最近，在沿海海洋底栖环境中发现了新的古细菌系统发育谱系。我们调查的相对丰度，垂直分布，系统发育组成，和空间变异ofdichroea在深海沉积物中收集的几个站在大西洋。定量寡核苷酸杂交实验表明，在深海沉积物（1500米深）的古菌16 S rRNA的相对丰度范围从约2.5%至8%的总原核rRNA。从10个深度间隔从沉积物芯收集在1,500，2,600，和4,500米的深度PCR扩增的古细菌rRNA基因（rDNA）的克隆文库构建。rDNA序列的系统发育分析显示，存在一个复杂的古菌种群结构，其成员可以分为两个王国，Crenarchaeota和Euryarchaeota离散的系统发育谱系。比较变性梯度凝胶电泳分析的古细菌16 S rDNA V3片段显示了显着的深度相关的变化，古细菌种群的组成。
ABSTRACT During the past few years Archaea have been recognized as a widespread and significant component of marine picoplankton assemblages and, more recently, the presence of novel archaeal phylogenetic lineages has been reported in coastal marine benthic environments. We investigated the relative abundance, vertical distribution, phylogenetic composition, and spatial variability ofArchaea in deep-sea sediments collected from several stations in the Atlantic Ocean. Quantitative oligonucleotide hybridization experiments indicated that the relative abundance of archaeal 16S rRNA in deep-sea sediments (1500 m deep) ranged from about 2.5 to 8% of the total prokaryotic rRNA. Clone libraries of PCR-amplified archaeal rRNA genes (rDNA) were constructed from 10 depth intervals obtained from sediment cores collected at depths of 1,500, 2,600, and 4,500 m. Phylogenetic analysis of rDNA sequences revealed the presence of a complex archaeal population structure, whose members could be grouped into discrete phylogenetic lineages within the two kingdoms, Crenarchaeota and Euryarchaeota. Comparative denaturing gradient gel electrophoresis profile analysis of archaeal 16S rDNA V3 fragments revealed a significant depth-related variability in the composition of the archaeal population.