Highly reactive cysteine residues in rodent hemoglobins
Highly reactive cysteine residues in rodent hemoglobins
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DOI：
10.1006/bbrc.2000.3326
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发表时间：
2000-08-28
影响因子：
3.1
通讯作者：
Miranda, JJ
中科院分区：
文献类型：
--
作者：
Miranda, JJ
Two hemoglobins with cysteine residues highly reactive toward electrophiles have been identified and characterized. Cys-125 beta of guinea pig hemoglobin has a low pK(a) and forms conjugates with electrophiles more quickly than glutathione and several orders of magnitude more quickly than other protein thiols. This cysteine is capable of intercepting benzoquinone, a known carcinogenic metabolite, before other protein nucleophiles can be modified. Cys-13 beta of mouse hemoglobin was observed to conjugate with electrophiles as quickly as glutathione. The structural basis of reactivity is different in the two hemoglobins and is analyzed in terms of hydrogen-bonding, solvent accessibility, and helix-dipole contributions. Complementing a previously characterized highly reactive cysteine in rat hemoglobin, identification of these cysteines suggests that the reactivity of these hemoglobins could represent a common function as a detoxification sink against carcinogens. (C) 2000 Academic Press.
