Sensitive and multiplexed detection of antibiotics using a suspension array platform based on silica-agarose hybrid microbeads.
Sensitive and multiplexed detection of antibiotics using a suspension array platform based on silica-agarose hybrid microbeads.
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DOI：
10.1016/j.jhazmat.2019.03.081
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发表时间：
2019-07
影响因子：
13.6
通讯作者：
Xuan Wang;Junhui Gong;Beilei Yuan;Zhaofang Chen;Juncheng Jiang
中科院分区：
文献类型：
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作者：
Xuan Wang;Junhui Gong;Beilei Yuan;Zhaofang Chen;Juncheng Jiang
建立了一种基于硅胶-琼脂糖杂交微球（SAHMs）的抗生素多重悬浮阵列检测平台。以氯霉素（CAP）、磺胺甲恶唑（SMX）、甲硝唑（MTZ）和阿莫西林（AMX）为模型化合物。将抗原（与BSA缀合的抗生素）固定在四种不同类型的SAHM的表面上。基于间接竞争免疫分析，通过多种抗生素与抗原之间特异性单克隆抗体的竞争来检测所选抗生素。由于SAHMs对非特异性蛋白质吸收的高抵抗性，该方法具有宽的线性范围（CAP为0.4 ~ 72.9 ng/mL，SMX为2.0 ~ 108.5 ng/mL，MTZ为2.6 ~ 142.2 ng/mL，AMX为1.0 ~ 63.3 ng/mL）和低检测限（0.09 ~ 0.8 ng/mL）。加标自来水样品的回收率为82%至113%，相对标准偏差低于14%，证明了所开发的方法进行测量的准确性。该方法具有高通量、灵活、准确的特点，为抗生素的同时检测提供了良好的平台。
A multiplex suspension array detection platform of antibiotics has been developed based on silica-agarose hybrid microbeads (SAHMs). Chloramphenicol (CAP), sulfamethoxazole (SMX), metronidazole (MTZ) and amoxicillin (AMX) were employed as model analytes. The antigens (the antibiotics conjugated with BSA) were immobilized on the surface of four different types of SAHMs. Based on an indirect competition immunoassay, the selected antibiotics are detected through the competition of the specific monoclonal antibodies between the multiple antibiotics and the antigens. Due to high resistance to nonspecific protein absorption of SAHMs, the proposed method exhibited wide linear ranges (0.4˜72.9 ng/mL for CAP, 2.0˜108.5 ng/mL for SMX, 2.6˜142.2 ng/mL for MTZ, 1.0˜63.3 ng/mL for AMX) and low detection limits of 0.09˜0.8 ng/mL. Recoveries for spiked tap water samples were from 82% to 113%, with relative standard deviation lower than 14%, demonstrating the accuracy of the measurements performed with the developed method. This work offered a high-throughput, flexible and accurate tool, which provides a good platform for simultaneous detection of antibiotics.