Preparation of affinity adsorbents and purification of lectins from natural sources.
Preparation of affinity adsorbents and purification of lectins from natural sources.
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亲和吸附剂的制备和天然来源凝集素的纯化。
DOI：
10.1007/978-1-4939-1292-6_5
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Ogawa H.
中科院分区：
文献类型：
--
作者：
Date K.;Ogawa H.
凝集素通过亲和色谱法纯化，以利用它们的碳水化合物特异性相互作用。高效的亲和吸附剂是获得具有不同特异性的均一凝集素的有力工具。在这里，我们描述了三种方法来制备亲和吸附剂，通过固定碳水化合物或糖缀合物的琼脂糖凝胶珠。由于配体在温和条件下通过稳定的非离子键固定化，因此吸附剂对凝集素具有高结合能力，非特异性吸附低，并且可以承受重复使用。这些程序既不需要专门的技术和设备，也不需要剧毒化合物。使用这些吸附剂，许多植物和动物凝集素可以在几个步骤中纯化。
Lectins are purified by affinity chromatography to take advantage of their carbohydrate-specific interactions. Highly efficient affinity adsorbents are powerful tools to obtain homogeneous lectins with distinct specificities. Here, we describe three methods to prepare affinity adsorbents by immobilizing carbohydrates or glycoconjugates on agarose gel beads. Because the ligands are immobilized via a stable and nonionic linkage under mild conditions, the adsorbents possess high binding capacity for lectins with low nonspecific adsorption and can withstand repeated use. The procedures require neither specialized techniques and apparatus nor highly toxic compounds. Using these adsorbents, many plant and animal lectins can be purified in a few steps.