JASMONATE-ZIM DOMAIN proteins engage Polycomb chromatin modifiers to modulate Jasmonate signaling in Arabidopsis
JASMONATE-ZIM DOMAIN proteins engage Polycomb chromatin modifiers to modulate Jasmonate signaling in Arabidopsis
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DOI：
10.1016/j.molp.2021.03.001
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发表时间：
2021-05-03
期刊：
影响因子：
27.5
通讯作者：
He, Yuehui
中科院分区：
文献类型：
--
作者：
Li, Zicong;Luo, Xiao;He, Yuehui
茉莉酸（JA）调节植物生长发育和胁迫反应的各个方面，在雄性生殖发育和防御食草动物和坏死型病原体中发挥重要作用。JAZ蛋白是JA信号通路中的关键调节因子，具有抑制JA应答基因表达的功能。在这里，我们表明，JAZ蛋白直接与几个染色质相关的Polycomb蛋白介导抑制性染色质修饰在JA响应基因，因此，他们在拟南芥中的转录抑制。遗传分析表明，发育缺陷，包括花药和花粉异常，导致的损失或JA信号的阻断部分挽救的损失Polycomb蛋白介导的染色质沉默（Polycomb抑制）。我们进一步发现，在花药和花粉发育过程中，JAZ介导的转录抑制需要Polycomb蛋白在四个关键的调控位点。对Polycomb因子的全基因组占有率和响应于JA的转录组重编程的分析表明，Polycomb阻遏参与了各种JA响应基因的阻遏。总之，我们的研究揭示了一个重要的染色质为基础的机制JAZ介导的转录抑制和JA信号在植物中。
Jasmonate (JA) regulates various aspects of plant growth and development and stress responses, with prominent roles in male reproductive development and defenses against herbivores and necrotrophic pathogens. JASMONATE-ZIM DOMAIN (JAZ) proteins are key regulators in the JA signaling pathway and function to repress the expression of JA-responsive genes. Here, we show that JAZ proteins directly interact with several chromatin-associated Polycomb proteins to mediate repressive chromatin modifications at JA-responsive genes and, thus, their transcriptional repression in Arabidopsis. Genetic analyses revealed that the developmental defects, including anther and pollen abnormalities, resulting from loss or block of JA signaling were partially rescued by loss of Polycomb protein-mediated chromatin silencing (Polycomb repression). We further found that JAZ-mediated transcriptional repression during anther and pollen development requires Polycomb proteins at four key regulatory loci. Analysis of genome-wide occupancy of a Polycomb factor and transcriptome reprogramming in response to JA revealed that Polycomb repression is involved in the repression of various JA-responsive genes. Taken together, our study reveals an important chromatin-based mechanism for JAZ-mediated transcriptional repression and JA signaling in plants.