SIMPLE METHOD FOR DNA RESTRICTION SITE MAPPING
SIMPLE METHOD FOR DNA RESTRICTION SITE MAPPING
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DOI：
10.1093/nar/3.9.2387
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发表时间：
1976-01-01
影响因子：
14.9
通讯作者：
BIRNSTIEL, ML
中科院分区：
文献类型：
--
作者：
SMITH, HO;BIRNSTIEL, ML
当一端用32P酶标记的DNA分子用限制性内切酶部分消化时，得到标记的DNA片段，这些片段形成一系列重叠的分子，所有分子都具有共同的标记末端。然后可以通过分析这些分子的大小分布来构建限制性图谱。该技术已被用于克隆组蛋白DNA（h22）的限制性酶切位点作图，其中在单个实验中可以准确地确定多达35个切割位点。
When a DNA molecule, enzymatically labelled with32P at one end, is partially digested with a restriction enzyme labelled DNA fragments are obtained which form an overlapping series of molecules, all with a common labelled terminus. A restriction map can then be constructed from an analysis of the size distribution of these molecules. This technique has been used for the restriction site mapping of cloned histone DNA (h22) where as many as 35 cleavage sites may be accurately determined in a single experiment.