Adenyl Cyclase of Cultured Mammalian Cells: Activation by Catecholamines
Adenyl Cyclase of Cultured Mammalian Cells: Activation by Catecholamines
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培养哺乳动物细胞的腺苷酸环化酶：儿茶酚胺的激活
作者：
M. Makman
Chang的肝细胞和3T6小鼠胚胎成纤维细胞含有大量的儿茶酚胺敏感的腺苷酸环化酶，而HeLa细胞含有相对较低的活性。肾上腺素和氟离子都能刺激每种细胞系的活性。与正常肝脏相比，Chang的肝细胞对肾上腺素的反应更大，而胰高血糖素没有可检测到的刺激。
Chang's liver cells and 3T6 mouse embryo fibroblasts contain high amounts of catecholamine-sensitive adenyl cyclase, whereas HeLa cells contain relatively low amounts of activity. Both epinephrine and fluoride ion stimulate activity of each cell line. In contrast to normal liver, Chang's liver cells show greater response to epinephrine and no detectable stimulation by glucagon.