Origin of nanomechanical cantilever motion generated from biomolecular interactions.
Origin of nanomechanical cantilever motion generated from biomolecular interactions.
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DOI：
10.1073/pnas.98.4.1560
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发表时间：
2001-02
影响因子：
11.1
通讯作者：
G. Wu;H. Ji;K. Hansen;T. Thundat;R. Datar;R. Cote;M. Hagan;A. Chakraborty;A. Majumdar
中科院分区：
文献类型：
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作者：
G. Wu;H. Ji;K. Hansen;T. Thundat;R. Datar;R. Cote;M. Hagan;A. Chakraborty;A. Majumdar
从生物分子相互作用产生纳米机械悬臂梁运动可以具有广泛的应用，从高通量生物分子检测到生物致动。虽然有人认为这种运动是由悬臂梁的表面应力变化引起的，但表面应力变化的起源至今尚未阐明。通过使用DNA杂交实验，我们表明，运动的起源在于在特定的生物分子相互作用引起的构型熵和分子间能量学的变化之间的相互作用。通过控制DNA杂交过程中的熵变，可以控制悬臂梁的运动方向。这些热力学原理也被用来解释蛋白质-配体结合产生的运动的起源。
Generation of nanomechanical cantilever motion from biomolecular interactions can have wide applications, ranging from high-throughput biomolecular detection to bioactuation. Although it has been suggested that such motion is caused by changes in surface stress of a cantilever beam, the origin of the surface-stress change has so far not been elucidated. By using DNA hybridization experiments, we show that the origin of motion lies in the interplay between changes in configurational entropy and intermolecular energetics induced by specific biomolecular interactions. By controlling entropy change during DNA hybridization, the direction of cantilever motion can be manipulated. These thermodynamic principles were also used to explain the origin of motion generated from protein-ligand binding.