The effects of a high-fat sucrose diet on functional outcome following cortical contusion injury in the rat
The effects of a high-fat sucrose diet on functional outcome following cortical contusion injury in the rat
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DOI：
10.1016/j.bbr.2011.04.028
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发表时间：
2011-09-30
影响因子：
2.7
通讯作者：
Heck, Sarah E.
中科院分区：
文献类型：
--
作者：
Hoane, Michael R.;Swan, Alicia A.;Heck, Sarah E.
创伤性脑损伤（TBI）是一个主要的公共卫生问题，每年影响170万美国人，其中约50，000人是致命的。高脂肪蔗糖（HFS）饮食是另一个公共卫生问题，可导致肥胖，高血压和许多其他衰弱性疾病。这两种疾病结合在一起可能会导致更复杂的问题。最近的研究表明，HFS饮食可以降低脑源性神经营养因子（BDNF）的水平，导致神经元和行为可塑性的降低。BDNF的这种减少被怀疑增加了大脑对损伤的易感性。为了测试HFS饮食对TBI后功能恢复的影响，在本研究中使用雄性Sprague-Dawley大鼠。在TBI前8周，将大鼠置于特殊HFS饮食（n = 14）或标准啮齿动物饮食（n =14）。在这八周的时间后，大鼠准备双侧额叶皮质挫伤损伤（CCI）或假手术。从TBI后两天开始，对动物进行一系列行为测试，以评估Morris水迷宫中的体感功能障碍和空间记忆，以及参考记忆和工作记忆任务。在测试之后，处死动物，并处理它们的大脑用于病变分析。在假手术动物中，HFS饮食使双侧触觉粘合剂去除试验的性能恶化。与HFS饮食的动物相比，标准饮食的受伤动物在粘合剂去除测试中的体感表现有更大的改善，并且在工作记忆任务上有更好的表现。HFS饮食还导致CC后皮质组织的损失显著大于标准饮食组。这项研究可能有助于确定营养特征或习惯如何与大脑损伤相互作用。(C)2011爱思唯尔有限公司版权所有。
Traumatic brain injury (TBI) is a major public health issue affecting 1.7 million Americans each year, of which approximately 50,000 are fatal. High-fat sucrose (HFS) diets are another public health issue which can lead to obesity, hypertension, and many other debilitating disorders. These two disorders combined can lead to more complicated issues. It has recently been shown that HFS diets can reduce levels of brain-derived neurotrophic factor (BDNF) leading to reductions in neuronal and behavioral plasticity. This reduction in BDNF is suspected of increasing the susceptibility of the brain to injury. To test the effects of a HFS diet on recovery of function post-TBI, male Sprague-Dawley rats were used in this study. Eight weeks prior to TBI, rats were placed on a special HFS diet (n = 14) or a standard rodent diet (n =14). Following this eight-week period, rats were prepared with bilateral frontal cortical contusion injuries (CCI) or sham procedures. Beginning two days post-TBI, animals were tested on a battery of behavioral tests to assess somatosensory dysfunction and spatial memory in the Morris water maze, with a reference memory and a working memory task. Following testing, animals were sacrificed and their brains processed for lesion analysis. The HFS diet worsened performance on the bilateral tactile adhesive removal test in sham animals. Injured animals on the Standard diet had a greater improvement in somatosensory performance in the adhesive removal test and had better performance on the working memory task compared to animals on the HFS diet. The HFS diet also resulted in significantly greater loss of cortical tissue post-CC than in the Standard diet group. This study may aid in determining how nutritional characteristics or habits interact with damage to the brain. (C) 2011 Elsevier B.V. All rights reserved.