Characterization of the L. manihotivorans α-Amylase Gene
Characterization of the L. manihotivorans α-Amylase Gene
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DOI：
10.3109/10425170109042048
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发表时间：
2001-01
期刊：
影响因子：
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通讯作者：
J. Morlon‐Guyot;F. Mucciolo-Roux;R. R. Sanoja-R.;J. Guyot
中科院分区：
文献类型：
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作者：
J. Morlon‐Guyot;F. Mucciolo-Roux;R. R. Sanoja-R.;J. Guyot
根据L. amylovorus CIP 102989和L. plantarum A6（Giraud和Cuny 1997）。成功地应用于L.已对食蟹猴LMG 18010 T菌株的一个2842 bp的区域进行了测序，该区域包含整个基因（2706 bp）及其推定的启动子。与L. amylovorus和L. plantarum amyA基因。推导的氨基酸序列与L. amylovorus和L. plantarum的α-淀粉酶，与B的α-淀粉酶的同源性分别为65%和59%。subtilis和S.牛，分别。L.食蟹猴LMG 18010 TamyA基因含有4个重复序列（SRU）。三种乳酸杆菌的amyA基因主要在SRU的数量和SRU侧翼区域的大小上不同。
Primers and probes were established from the sequences of the α-amylase genes (amyA) of L. amylovorus CIP 102989 and of L. plantarum A6 (Giraud and Cuny 1997). They were successfully used for the detection of the amy A gene in L. manihotivorans strain LMG 18010T and a 2842 bp region, containing the entire gene (2706 bp) with its putative promoter has been sequenced. More than 98% nucleotide sequence identities was found with L. amylovorus and L. plantarum amyA genes. The deduced amino acid sequence shares more than 96% amino acid sequence identities with L. amylovorus and L. plantarum ct-amy-lases, and also 65% and 59% identities with the cc-amy-lases of B. subtilis and S. bovis, respectively. The 3′ terminal part of L. manihotivorans LMG 18010TamyA gene contained four repeated sequences (SRU). The amyA genes of the three lactobacilli species differed mainly in the number of SRU and in the size of the flanking regions of the SRU.