A bZIP factor, TRAB1, interacts with VP1 and mediates abscisic acid-induced transcription
A bZIP factor, TRAB1, interacts with VP1 and mediates abscisic acid-induced transcription
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DOI：
10.1073/pnas.96.26.15348
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发表时间：
1999-12-21
影响因子：
11.1
通讯作者：
Hattori, T
中科院分区：
文献类型：
--
作者：
Hobo, T;Kowyama, Y;Hattori, T
转录因子VP1通过激活响应于胁迫激素脱落酸（阿坝）的基因来调节植物种子的成熟和休眠。虽然激活涉及ABA反应元件（ABRE），但VP1本身并不特异性结合ABRE。相反，我们已经确定并克隆了一个碱性区域亮氨酸拉链（bZIP）因子，TRAB1，与VP1和ABRE相互作用。如果细胞表达GAL4 DNA结合结构域：：TRAB1融合蛋白，则从具有GAL4结合位点的嵌合启动子的转录是ABA诱导的。结果表明，TRAB1是一个真正的反式作用因子参与ABA调控的转录，并揭示了一个分子机制的VP1依赖，ABA诱导的转录，控制成熟和休眠在植物胚。
The transcription factor VP1 regulates maturation and dormancy in plant seeds by activating genes responsive to the stress hormone abscisic acid (ABA). Although activation involves ABA-responsive elements (ABREs), VP1 itself does not specifically bind ABREs. Instead, we have identified and cloned a basic region leucine zipper (bZIP) factor, TRAB1, that interacts with both VP1 and ABREs. Transcription from a chimeric promoter with GAL4-binding sites was ABA-inducible if cells expressed a GAL4 DNA-binding domain::TRAB1 fusion protein. Results indicate that TRAB1 is a true trans-acting factor involved in ABA-regulated transcription and reveal a molecular mechanism for the VP1-dependent, ABA-inducible transcription that controls maturation and dormancy in plant embryos.