Dissecting sex pheromone communication of Mythimna separata (Walker) in North China from receptor molecules and antennal lobes to behavior
Dissecting sex pheromone communication of Mythimna separata (Walker) in North China from receptor molecules and antennal lobes to behavior
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从受体分子、触角叶到行为剖析华北粘虫性信息素通讯
DOI：
10.1016/j.ibmb.2019.103176
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发表时间：
2019-08-01
影响因子：
3.8
通讯作者：
Wang, Chen-Zhu
中科院分区：
文献类型：
--
作者：
Jiang, Nan-Ji;Tang, Rui;Wang, Chen-Zhu
粘虫释放地理种群特异性信息素，主要成分为Z11-16：A1d或Z11-16：Ac。本研究综合运用电生理、行为和遗传分析方法，从信息素受体、触角叶到行为三个方面研究了北中国地区粘虫的性信息素传递。GC-EAD结果表明，Z11-16：A1d是唯一能在信息素腺体提取物中引起电生理反应的化合物。进一步的体内光学成像研究表明，Z11-16：A1d激活了MGC的堆积，并表现出剂量依赖的反应。风洞试验表明，仅Z11-16：A1d就足以诱导整个男性性行为序列。转录组和Q-PCR结果表明，MSepOR3在雄性触角中特异而丰富地表达。通过非洲爪哇卵母细胞和双电极电压钳记录，我们最终验证了表达MSepOR3/Orco的卵母细胞对Z11-16：A1d有剂量依赖性的反应。我们建议用Z11-16：A1d单倍体对中国北部地区的分离线虫种群进行监测。
The Oriental armyworm, Mythimna separata, has been described to emit geographic population-specific sex pheromones, with either Z11-16:A1d or Z11-16:Ac as the major component. Using a comprehensive set of electrophysiological, behavioral, and genetic analyses, we study the sex pheromone communication of M. separata in North China from pheromone receptors and antennal lobe to behavior. GC-EAD results show that Z11-16:A1d is the only compound eliciting electrophysiological responses in pheromone gland extracts. Further in vivo optical imaging studies reveal that Z11-16:A1d activates the cumulus of the MGC and show dose-dependent responses. The wind tunnel tests demonstrate that Z11-16:A1d alone is sufficient to induce the entire sequence of male sexual behaviors. Transcriptome and q-PCR results show that MsepOR3 is specifically and abundantly expressed in male antennae. By using the Xenopus oocytes and two-electrode voltage-clamp recording, we finally validate that the oocytes expressing MsepOR3/ORco gave dose dependent responses to Z11-16:A1d. We suggest single Z11-16:A1d could be used for monitoring the population of M. separata in North China.