Interferon, double-stranded RNA and RNA degradation. Fractionation of the endonucleaseINT system into two macromolecular components; role of a small molecule in nuclease activation.
Interferon, double-stranded RNA and RNA degradation. Fractionation of the endonucleaseINT system into two macromolecular components; role of a small molecule in nuclease activation.
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干扰素、双链RNA和RNA降解。
DOI：
10.1016/0006-291x(78)91443-2
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发表时间：
1978
影响因子：
3.1
通讯作者：
P. Lengyel
中科院分区：
文献类型：
--
作者：
L. Ratner;Roger C. Wiegand;P. J. Farrell;G. Sen;B. Cabrer;P. Lengyel
我们之前报道过，a)用双链RNA和ATP孵化干扰素处理过的埃利希腹水肿瘤细胞的提取物可激活一种内切酶，b)激活后，双链RNA和ATP可被降解而不损害内切酶的活性。本文报道了该过程中涉及的两种大分子成分（de1int和DE2INT）的分离和部分纯化。在与双链RNA和ATP成分孵育后，de1intd产生低分子量的热稳定产物（称为核酸酶激活剂）。与核酸酶激活剂孵育后，de2in2组分中的潜伏核酸酶被激活。
We reported earlier that a) the incubation of an extract from interferon-treated Ehrlich ascites tumor cells with double-straded RNA and ATP results in the activation of an endonuclease and b) after the activation the double-stranded RNA and ATP can be degraded without impairing the activity of the endonuclease. We report now the separation and partial purification of two macromolecular components (DE1INTand DE2INT) involved in the process. Upon incubation with double-stranded RNA and ATP component DE1INTgenerates a heat-stable product of low molecular weight (designated as nuclease activator). On incubation with the nuclease activator a latent nuclease in component DE2INTis activated.