Potential role of entactin in hemostasis. Specific interaction of entactin with fibrinogen A alpha and B beta chains.
Potential role of entactin in hemostasis. Specific interaction of entactin with fibrinogen A alpha and B beta chains.
复制标题
DOI：
10.1016/s0021-9258(18)55134-6
复制
发表时间：
1991-10
期刊：
影响因子：
--
通讯作者：
C. Wu;A. Chung
中科院分区：
文献类型：
--
作者：
C. Wu;A. Chung
内皮基底膜、血小板和凝血因子之间的相互作用在止血中起着至关重要的作用。Entactin是基底膜不可或缺的普遍成分。实验旨在研究entactin和凝血因子之间的相互作用。我们首次证明了entactin以一种特定的方式与纤维蛋白原相互作用。Entactin结合位点定位于纤维蛋白原的Aα和Bβ链上。Entactin与Aα链或Bβ链的结合不依赖于二价阳离子。35S标记的entactin与固定的纤维蛋白原Aα或Bβ链的结合是浓度依赖且饱和的，可被未标记的entactin、可溶性纤维蛋白原、抗entactin抗血清或抗纤维蛋白原抗血清所抑制。此外，我们提供的证据表明，转谷氨酰胺酶可以使entactin与自身，也可能与纤维蛋白(原)发生交叉连接。这些新的性质，以及它的细胞结合、趋化和促进吞噬活性以及在基底膜中的普遍分布，表明entactin可能在止血和伤口愈合中发挥重要作用。
The interactions between the endothelial basement membrane, platelets, and coagulation factors play essential roles in hemostasis. Entactin is an integral and ubiquitous component of the basement membrane. Experiments were designed to study the interactions between entactin and blood coagulation factors. We have demonstrated, for the first time, that entactin interacts with fibrinogen in a specific manner. Entactin binding sites have been localized to the A alpha and B beta chains of fibrinogen. The binding of entactin to either the A alpha chain or the B beta chain was divalent cation-independent. The binding of 35S-labeled entactin to the immobilized fibrinogen A alpha or B beta chain was concentration-dependent and saturable and could be inhibited by unlabeled entactin, soluble fibrinogen, anti-entactin antiserum, or anti-fibrinogen antiserum. In addition, we have provided evidence that entactin can be cross-linked to itself, and probably also to fibrin(ogen), by transglutaminase. These novel properties, together with its cell binding, chemotactic, and phagocytic promoting activities and ubiquitous distribution in basement membranes, suggest that entactin may play important roles in hemostasis and wound healing.